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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents



  history
ID: 251366.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
Essential role of the V-ATPase in male gametophyte development
Authors:Dettmer, J.; Schubert, D.; Calvo-Weimar, O.; Stierhof, Y. D.; Schmidt, R.; Schumacher, K.
Language:English
Date of Publication (YYYY-MM-DD):2005-01
Title of Journal:Plant Journal
Journal Abbrev.:Plant J
Volume:41
Issue / Number:1
Start Page:117
End Page:124
Review Status:not specified
Audience:Not Specified
Abstract / Description:Intracellular pH homeostasis is a prerequisite for biological processes and requires the action of proton pumps. The vacuolar H+-ATPase (V-ATPase) is involved in regulating pH in endomembrane compartments of all eukaryotic cells. In plants, there is an additional endomembrane proton pump, H+-pyrophosphatase (H+-PPase). However, the relative roles of the two types of pumps in endomembrane acidification and energization of secondary active transport are unclear. Here, we show that a strong T-DNA insertion allele of VHA-A, the single copy gene encoding the catalytic subunit of the Arabidopsis V-ATPase, causes complete male and partial female gametophytic lethality. Severe changes in the morphology of Golgi stacks and Golgi-derived vesicles in male gametophytes are the first visible symptoms of cell degeneration leading to a failure to develop mature pollen. Similar effects on Golgi morphology were observed in pollen tubes when growth was blocked by Concanamycin A, a specific V-ATPase inhibitor. Taken together, our results suggests that V-ATPase function is essential for Golgi organization and development of the male gametophyte.
Free Keywords:v-atpase
; golgi
; gametogenesis
; arabidopsis
; vacuolar h+-atpase
; arabidopsis-thaliana
; golgi-apparatus
; proton pumps
; plant-growth
; subunit-c
; genes
; cells
; organelles
; reveals
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie/Unabhängige Forschungsgruppen/AG Schmidt
External Affiliations:Univ Tubingen, ZMBP Plant Physiol, Morgenstelle 1, D-72076 Tubingen, Germany
Univ Tubingen, ZMBP Plant Physiol, D-72076 Tubingen, Germany
Max Planck Inst Mol Physiol, D-14424 Potsdam, Germany
Univ Tubingen, ZMBP Microscopy, D-72076 Tubingen, Germany
Identifiers:ISI:000225792900010 [ID No:1]
ISI:000225792900010 [ID No:2]
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