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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents



  history
ID: 251369.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
Expression of an Escherichia coli phosphoglucomutase in potato (Solanum tuberosum L.) results in minor changes in tuber metabolism and a considerable delay in tuber sprouting
Authors:Lytovchenko, A.; Hajirezaei, M.; Eickmeier, I.; Mittendorf, V.; Sonnewald, U.; Willmitzer, L.; Fernie, A. R.
Language:English
Date of Publication (YYYY-MM-DD):2005-08
Title of Journal:Planta
Journal Abbrev.:Planta
Volume:221
Issue / Number:6
Start Page:915
End Page:927
Review Status:not specified
Audience:Not Specified
Abstract / Description:The aim of this work was to evaluate the influence of elevating the cytosolic activity of phosphoglucomutase (PGM; EC 5.4.2.2) on photosynthesis, growth and heterotrophic metabolism. Here we describe the generation of novel transgenic plants expressing an Escherichia coli phosphoglucomutase (EcPGM) under the control of the 35S promoter. These lines were characterised by an accumulation of leaf sucrose, despite displaying no alterations in photosynthetic carbon partitioning, and a reduced tuber starch content. Determinations of the levels of a wide range of other metabolites revealed dramatic reductions in maltose and other sugars in leaves of the transformants, as well as a modification of the pattern of organic and amino acid content in tubers of these lines. Intriguingly, the transgenics also displayed a dramatically delayed rate of sprouting and significantly enhanced rate of respiration, however, it is important to note that the severity of these traits did not always correlate with the level of transgene expression. These results are discussed in the context of current understanding of the control of respiration and the breaking of tuber dormancy.
Free Keywords:carbohydrate metabolism
; phosphoglucomutase
; glycolysis
; solanum tuberosum
; starch synthesis
; low internal oxygen
; plastidial phosphoglucomutase
; carbohydrate-metabolism
; cytosolic phosphoglucomutase
; sucrose phosphorylase
; antisense inhibition
; aldolase activity
; starch synthesis
; gas-exchange
; wild-type
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie/Molekulare Physiologie/AG Willmitzer/Fernie
External Affiliations:Max Planck Inst Mol Pflanzenphysiol, Dept Willmitzer, Muhlenberg 1, D-14476 Golm, Germany
Max Planck Inst Mol Pflanzenphysiol, Dept Willmitzer, D-14476 Golm, Germany
Inst Pflanzengenet & Kulturpflanzenforsch, D-06466 Gatersleben, Germany
Identifiers:ISI:000231313200017 [ID No:1]
ISI:000231313200017 [ID No:2]
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