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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents

ID: 251444.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
Metabolome analysis: the potential of in vivo labeling with stable isotopes for metabolite profiling
Authors:Birkemeyer, C.; Luedemann, A.; Wagner, C.; Erban, A.; Kopka, J.
Date of Publication (YYYY-MM-DD):2005-01
Title of Journal:Trends in Biotechnology
Journal Abbrev.:Trends Biotechnol
Issue / Number:1
Start Page:28
End Page:33
Review Status:not specified
Audience:Not Specified
Abstract / Description:Metabolome analysis technologies are still in early development because, unlike genome, transcriptome and proteome analyses, metabolome analysis has to deal with a highly diverse range of biomolecules. Combinations of different analytical platforms are therefore required for comprehensive metabolomic studies. Each of these platforms covers only part of the metabolome. To establish multiparallel technologies, thorough standardization of each measured metabolite is required. Standardization is best achieved by addition of a specific stable isotope-labeled compound, a mass isotopomer, for each metabolite. This suggestion, at first glance, seems unrealistic because of cost and time constraints. A possible solution to this problem is discussed in this article. Saturation in vivo labeling with stable isotopes enables the biosynthesis of differentially mass-labeled metabolite mixtures, which can be exploited for highly standardized metabolite profiling by mass isotopomer ratios.
Free Keywords:difference gel-electrophoresis
; ionization mass-spectrometry
; central carbon metabolism
; functional genomics
; gas-chromatography
; ratio analysis
; flux analysis
; tof-ms
; yeast
; quantification
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie/Molekulare Physiologie/AG Kopka
External Affiliations:Max Planck Inst Mol Plant Physiol, Dpet Willmitzer, Am Muhlenberg 1, D-14467 Golm, Germany
Max Planck Inst Mol Plant Physiol, Dpet Willmitzer, D-14467 Golm, Germany
Identifiers:ISI:000226565700009 [ID No:1]
ISI:000226565700009 [ID No:2]
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