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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents



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ID: 251459.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
Profiling of diurnal patterns of metabolite and transcript abundance in potato (Solanum tuberosum) leaves
Authors:Urbanczyk-Wochniak, E.; Baxter, C.; Kolbe, A.; Kopka, J.; Sweetlove, L. J.; Fernie, A. R.
Language:English
Date of Publication (YYYY-MM-DD):2005-08
Title of Journal:Planta
Journal Abbrev.:Planta
Volume:221
Issue / Number:6
Start Page:891
End Page:903
Review Status:not specified
Audience:Not Specified
Abstract / Description:Diurnal changes in carbohydrates and a broad range of primary metabolites were analysed through a diurnal period in potato leaves (Solanum tuberosum cv. Desiree) using an established gas chromatography-mass spectrometry based metabolic profiling protocol alongside conventional spectrophotometric technologies. In tandem, we profiled transcript levels using both a custom array containing approximately 2,500 cDNA clones predominantly representing transcripts involved in primary metabolism and commercially available arrays containing approximately 12,000 cDNA clones that gave coverage of transcript levels over a broader functional range. The levels of many metabolites and transcripts varied during the diurnal period with 56 significant differences observed in the metabolite contents and 832 significant differences recorded in transcript levels. Whilst a large number of the differences would be expected from what has been known previously, several novel changes were observed in these experiments. Notably, qualitative comparison of the combined data sets obtained from the parallel analysis of transcripts and metabolites suggests relatively few changes in gene expression strongly correlate with changes in metabolite levels during a diurnal cycle. Furthermore, these changes appear to be confined to the central metabolic pathways. However, principal component analysis of the metabolic profiles obtained here revealed that metabolite patterns change progressively through a diurnal period suggesting the operation of mechanisms for tight temporal regulation of metabolite composition.
Free Keywords:diurnal changes
; transcript profiling
; metabolic profiling
; solanum tuberosum
; photosynthetic carbon metabolism
; alpha-ketoglutarate aminotransferase
; transgenic arabidopsis plants
; sucrose-phosphate synthase
; nitrate reductase-activity
; gene-expression
; tobacco-leaves
; phosphoenolpyruvate carboxylase
; fructose 2,6-bisphosphate
; isocitrate dehydrogenase
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie/Molekulare Physiologie/AG Kopka
MPI für molekulare Pflanzenphysiologie/Molekulare Physiologie/AG Willmitzer/Fernie
External Affiliations:Max Planck Inst Mol Pflanzenphysiol, Muhlenberg 1, D-14476 Golm, Germany
Max Planck Inst Mol Pflanzenphysiol, D-14476 Golm, Germany
Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
Identifiers:ISI:000231313200015 [ID No:1]
ISI:000231313200015 [ID No:2]
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