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          Institute: MPI für molekulare Pflanzenphysiologie     Collection: Publikationen Pflanzenphysiologie     Display Documents



  history
ID: 251469.0, MPI für molekulare Pflanzenphysiologie / Publikationen Pflanzenphysiologie
Systems rebalancing of metabolism in response to sulfur deprivation, as revealed by metabolome analysis of arabidopsis plants
Authors:Nikiforova, V. J.; Kopka, J.; Tolstikov, V.; Fiehn, O.; Hopkins, L.; Hawkesford, M. J.; Hesse, H.; Hoefgen, R.
Language:English
Date of Publication (YYYY-MM-DD):2005-05
Title of Journal:Plant Physiology
Journal Abbrev.:Plant Physiol
Volume:138
Issue / Number:1
Start Page:304
End Page:318
Review Status:not specified
Audience:Not Specified
Abstract / Description:Sulfur is an essential macroelement in plant and animal nutrition. Plants assimilate inorganic sulfate into two sulfur-containing amino acids, cysteine and methionine. Low supply of sulfate leads to decreased sulfur pools within plant tissues. As sulfur-related metabolites represent an integral part of plant metabolism with multiple interactions, sulfur deficiency stress induces a number of adaptive responses, which must be coordinated. To reveal the coordinating network of adaptations to sulfur deficiency, metabolite profiling of Arabidopsis has been undertaken. Gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry techniques revealed the response patterns of 6,023 peaks of nonredundant ion traces and relative concentration levels of 134 nonredundant compounds of known chemical structure. Here, we provide a catalogue of the detected metabolic changes and reconstruct the coordinating network of their mutual influences. The observed decrease in biomass, as well as in levels of proteins, chlorophylls, and total RNA, gives evidence for a general reduction of metabolic activity under conditions of depleted sulfur supply. This is achieved by a systemic adjustment of metabolism involving the major metabolic pathways. Sulfur/carbon/nitrogen are partitioned by accumulation of metabolites along the pathway O-acetylserine to serine to glycine, and are further channeled together with the nitrogen-rich compound glutamine into allantoin. Mutual influences between sulfur assimilation, nitrogen imbalance, lipid breakdown, purine metabolism, and enhanced photorespiration associated with sulfur-deficiency stress are revealed in this study. These responses may be assembled into a global scheme of metabolic regulation induced by sulfur nutritional stress, which optimizes resources for seed production.
Free Keywords:acetyl-l-serine
; mass-spectrometry
; gene-expression
; sulfate transporter
; gas-chromatography
; hordeum-vulgare
; nitrogen
; leaves
; growth
; photorespiration
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Pflanzenphysiologie/Molekulare Physiologie/AG Hoefgen
MPI für molekulare Pflanzenphysiologie/Molekulare Physiologie/AG Kopka
External Affiliations:Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany
Max Planck Inst Mol Plant Physiol, D-14476 Golm, Germany
Russian Acad Sci, KA Timiryazev Plant Physiol Inst, Moscow 127276, Russia
Rothamsted Res, Harpenden AL5 2JQ, Herts, England
Identifiers:ISI:000229023100031 [ID No:1]
ISI:000229023100031 [ID No:2]
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