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          Institute: MPI für evolutionäre Anthropologie     Collection: Publications MPI for Evolutionary Anthropology     Display Documents

ID: 251572.0, MPI für evolutionäre Anthropologie / Publications MPI for Evolutionary Anthropology
Optimal storage conditions for highly dilute DNA samples: A role for trehalose as a preserving agent
Authors:Smith, S.; Morin, Phillip A.
Date of Publication (YYYY-MM-DD):2005-09
Title of Journal:Journal of Forensic Sciences
Issue / Number:5
Start Page:1101
End Page:1108
Review Status:not specified
Audience:Not Specified
Abstract / Description:DNA extraction from trace samples or noninvasively collected samples often results in the recovery of low concentration solutions of DNA that are prone to DNA degradation or other loss. Because of the difficulty in obtaining such samples, and their potentially high value in wildlife and forensic studies, it is critical that optimal methods are employed for their long-term storage. We assessed the amplification yield of samples kept under different storage conditions with the addition of potential preserving agents. We stored dilutions of known concentration human placental DNA, and gorilla fecal DNA, under four conditions (+4 degrees C, -20 degrees C, -80 degrees C, dry at room temperature), and with three additives (Tris EDTA (TE) buffer, Hind III digested Lambda DNA, trehalose). The effectiveness of the treatment methods was tested at regular intervals using qPCR to assess the quantity of amplifiable DNA, and a PCR assay of a larger 757 by fragment to evaluate the quality of that remaining DNA. The highest quantity of DNA remained in samples stored at -80 degrees C, regardless of storage additives, and those dried at room temperature in the presence of trehalose. Surprisingly, DNA was best preserved in the presence of trehalose, either dried or at -80 degrees C; significant quality loss occurred with -20 degrees C and +4 degrees C storage. [References: 26]
Free Keywords:Forensic science
; Dna storage
; Dna degradation
; Trehalose
; Noninvasive samples
; Qpcr.
; Preservation methods
; Brown bear
; Fecal dna
; Pcr
; Amplification
; Extraction
; Stability
; Enzymes
; Length
; Hairs.
External Publication Status:published
Document Type:Article
Communicated by:Gisela Lausberg
Affiliations:MPI für evolutionäre Anthropologie/Department of Evolutionary Genetics
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