Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für biophysikalische Chemie     Collection: Molekulare Entwicklungsbiologie (Prof. Herbert Jäckle)     Display Documents



  history
ID: 256118.0, MPI für biophysikalische Chemie / Molekulare Entwicklungsbiologie (Prof. Herbert Jäckle)
Gain-of-Function Screen for Genes That Affect Drosophila Muscle Pattern Formation
Authors:Staudt, N.; Molitor, A.; Somogyi, K.; Mata, J.; Curado, S.; Eulenberg, K. G.; Meise, M.; Siegmund, T.; Haeder, T.; Hilfiker, A.; Broenner, G.; Ephrussi, A.; Rroth, P.; Cohen, S. M.; Fellert, S.; Chung, H. R.; Piepenburg, O.; Schaefer, U.; Jaeckle, H.; Vorbrueggen, G.
Language:English
Date of Publication (YYYY-MM-DD):2005
Title of Journal:PLoS Genetics
Volume:1
Issue / Number:4
Sequence Number of Article:e55
Copyright:© 2005 Staudt et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für biophysikalische Chemie/Abt. Herbert Jäckle / 170
MPI für biophysikalische Chemie/AG Gerd Vorbrüggen
External Affiliations:DeveloGen, Göttingen, Germany
Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany
Identifiers:DOI:10.1371/journal.pgen.0010055
Full Text:
You have privileges to view the following file(s):
PLoS_Genet_1(4)_e55.pdf  [1,00 Mb]   
 
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.