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          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents



  history
ID: 264875.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Gene expression analysis of pancreatic cell lines reveals genes overexpressed in pancreatic cancer
Authors:Aldinger, Ingo; Dittert, Dag; Peiper, Matthias; Fusco, Alberto; Chiappetta, Gennaro; Staub, Eike; Loehr, Matthias; Jesnowsk, Ralf; Baretton, Gustavo; Ockert, Detlef; Saeger, Hans-Detlev; Gruetzmann, Robert; Pilarsky, Christian
Language:English
Date of Publication (YYYY-MM-DD):2005-06-23
Title of Journal:Pancreatology : Official Journal of the International Association of Pancreatology (IAP), European Pancreatic Club
Journal Abbrev.:EPC
Volume:5
Issue / Number:4-5
Start Page:370
End Page:379
Copyright:© 2006 S. Karger AG, Basel
Review Status:not specified
Audience:Experts Only
Abstract / Description:Background: Pancreatic cancer is one of the leading causes of cancer-related death. Using DNA gene expression analysis based on a custom made Affymetrix cancer array, we investigated the expression pattern of both primary and established pancreatic carcinoma cell lines. Methods: We analyzed the gene expression of 5 established pancreatic cancer cell lines (AsPC-1, BxPC-3, Capan-1, Capan-2 and HPAF II) and 5 primary isolates, 1 of them derived from benign pancreatic duct cells. Results: Out of 1,540 genes which were expressed in at least 3 experiments, we found 122 genes upregulated and 18 downregulated in tumor cell lines compared to benign cells with a fold change >3. Several of the upregulated genes (like Prefoldin 5, ADAM9 and E-cadherin) have been associated with pancreatic cancer before. The other differentially regulated genes, however, play a so far unknown role in the course of human pancreatic carcinoma. By means of immunohistochemistry we could show that thymosin beta-10 (TMSB10), upregulated in tumor cell lines, is expressed in human pancreatic carcinoma, but not in non-neoplastic pancreatic tissue, suggesting a role for TMSB10 in the carcinogenesis of pancreatic carcinoma. Conclusion: Using gene expression profiling of pancreatic cell lines we were able to identify genes differentially expressed in pancreatic adenocarcinoma, which might contribute to pancreatic cancer development.
Free Keywords:Pancreas
Cancer
Cell line
Primary isolates
Microarray
Thymosin
Immunohistochemistry
External Publication Status:published
Document Type:Article
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:Department of Visceral-, Thoracic- and Vascular Surgery,University Hospital, Dresden, Germany;
Department of Pathology, University Hospital, Dresden, Germany;
Department of General and Visceral Surgery, University Hospital, Duesseldorf, Germany;
Centro di Endocrinologia ed Oncologia Sperimentale del CNR, c/o Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli Federico II, Naples,Italy;
Department of Experimental Oncology, Istituto dei Tumori, Naples, Italy;
metaGen Pharmaceuticals, Berlin, Germany,
Medical Department, University Hospital, Mannheim, Germany
Identifiers:DOI:10.1159/000086537
ISSN:1424-3903
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