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          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents



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ID: 264907.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Genomic analysis of Drosophila chromosome underreplication reveals a link between replication control and transcriptional territories
Authors:Belyakin, Stepan N.; Christophides, George K.; Alekseyenko, Artyom A.; Kriventseva, Evgenia V.; Belyaeva, Elena S.; Nanayev, Roman A.; Makunin, Igor V.; Hild, M.; Beckmann, B.; Haas, Stefan A.; Koch, B.; Solovyev, V.; Busold, C.; Fellenberg, K.; Boutros, M.; Vingron, Martin; Sauer, F.; Hoheisel, J. D.; Paro, R.
Language:English
Corporate body:Heidelberg Fly Array Consortium
Date of Publication (YYYY-MM-DD):2005-04-01
Title of Journal:Proceedings of the National Academy of Sciences
Journal Abbrev.:PNAS
Volume:107
Issue / Number:23
Start Page:8269
End Page:8274
Copyright:© 2005 by The National Academy of Sciences of the USA
Review Status:not specified
Audience:Experts Only
Abstract / Description:In Drosophila polytene chromosomes, most late-replicating regions remain underreplicated. A loss-of-function mutant of the suppressor of underreplication [Su(UR)] gene suppresses underreplication (UR), whereas extra copies of this gene enhance the level and number of regions showing UR. By combining DNA microarray analysis with manipulation of the number of Su(UR) gene copies, we achieved genomic-scale molecular identification of 1,036 genes that are arranged in clusters located in 52 UR chromosomal regions. These regions overlap extensively (96%) but are not completely identical with late-replicating regions of mitotically dividing Kc cells in culture. Reanalysis of published gene expression profiles revealed that genomic regions defined by replication properties include clusters of coordinately expressed genes. Genomic regions that are UR in polytene chromosomes and late replicated in Kc cell chromosomes show a particularly common association with transcriptional territories that are expressed in testis/males but not ovary/females or embryos. An attractive hypothesis for future testing is that factors involved in replication control, such as SU(UR), may interact physically with those involved in epigenetic silencing of transcription territories.
Free Keywords:DNA replication
polytene chromosomes
suppressor of underreplication
transcriptional silencing
Comment of the Author/Creator:Published online before print May 31, 2005
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:Institute of Cytology and Genetics of Siberian Division, Russian Academy of Sciences, Novosibirsk 630090, Russia; {dagger}European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany;
Zentrum für Molekulare Biologie, Ruprecht-Karls-Universitaet, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany;
Harvard Medical School–Partners Healthcare Center for Genetics and Genomics, 77 Louis Pasteur Avenue, Boston, MA 02115, USA;
Heidelberg Fly Array Consortium: M. Hilda, B. Beckmannb, S. A. Haasc, B. Kocha, V. Solovyevd, C. Busoldb, K. Fellenbergb, M. Boutrose, M. Vingron, F. Sauera,f, J. D. Hoheiselb, and R. Paroa, Heidelberg, Germany;
Zentrum für Molekulare Biologie Heidelberg, University of Heidelberg, Im Neuenheimer Feld 282, 69120 Heidelberg, Germany;
Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany;
Softberry, Inc., 116 Radio Circle, Suite 400, Mount Kisko, NY 10549, USA;
Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany;
Department of Biochemistry, University of California, Riverside, CA 92521, USA.
Identifiers:DOI:10.1073/pnas.0502702102
ISSN:1091-6490
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