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          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents



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ID: 265039.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Comparative promoter region analysis powered by CORG
Authors:Dieterich, Christoph; Grossmann, Steffen; Tanzer, Andrea; Röpcke, Stefan; Arndt, Peter F; Stadler, Peter F; Vingron, Martin
Language:English
Date of Publication (YYYY-MM-DD):2005-02-21
Title of Journal:BMC Gnomics
Volume:6
Start Page:24
End Page:24
Copyright:© 1999-2006 BioMed Central Ltd unless otherwise stated
Review Status:not specified
Audience:Experts Only
Abstract / Description:Background

Promoters are key players in gene regulation. They receive signals from various sources (e.g. cell surface receptors) and control the level of transcription initiation, which largely determines gene expression. In vertebrates, transcription start sites and surrounding regulatory elements are often poorly defined. To support promoter analysis, we present CORG http://corg.molgen.mpg.de, a framework for studying upstream regions including untranslated exons (5' UTR).

Description

The automated annotation of promoter regions integrates information of two kinds. First, statistically significant cross-species conservation within upstream regions of orthologous genes is detected. Pairwise as well as multiple sequence comparisons are computed. Second, binding site descriptions (position-weight matrices) are employed to predict conserved regulatory elements with a novel approach. Assembled EST sequences and verified transcription start sites are incorporated to distinguish exonic from other sequences.

As of now, we have included 5 species in our analysis pipeline (man, mouse, rat, fugu and zebrafish). We characterized promoter regions of 16,127 groups of orthologous genes. All data are presented in an intuitive way via our web site. Users are free to export data for single genes or access larger data sets via our DAS server http://tomcat.molgen.mpg.de:8080/das. The benefits of our framework are exemplarily shown in the context of phylogenetic profiling of transcription factor binding sites and detection of microRNAs close to transcription start sites of our gene set.

Conclusion

The CORG platform is a versatile tool to support analyses of gene regulation in vertebrate promoter regions. Applications for CORG cover a broad range from studying evolution of DNA binding sites and promoter constitution to the discovery of new regulatory sequence elements (e.g. microRNAs and binding sites).
Free Keywords:Database
External Publication Status:published
Document Type:Article
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:Institute for Theoretical Chemistry and Structural Biology, University of Vienna, Währingerstrasse 17, A-1090 Wien, Austria;
Bioinformatics Group, Department of Computer Science, University of Leipzig, Kreuzstrasse 7b, D-04103 Leipzig, Germany.
Identifiers:DOI:10.1186/1471-2164-6-24
ISSN:1471-2164
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