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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 268494.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Primary differentiation in the human blastocyst : comparative molecular portraits of inner cell mass and trophectoderm cells
Authors:Adjaye, James; Huntriss, John; Herwig, Ralf; BenKahla, Alia; Brink, Thore C.; Wierling, Christoph; Hultschig, Claus; Groth, Detlef; Yaspo, Marie-Laure; Picton, Helen M.; Gosden, Roger G.; Lehrach, Hans
Date of Publication (YYYY-MM-DD):2005-11-10
Title of Journal:Stem Cells
Issue / Number:10
Start Page:1514
End Page:1525
Copyright:© 2005 by AlphaMed Press
Review Status:not specified
Audience:Experts Only
Abstract / Description:The primary differentiation event during mammalian development occurs at the blastocyst stage and leads to the delineation of the inner cell mass (ICM) and the trophectoderm (TE). We provide the first global mRNA expression data from immunosurgically dissected ICM cells, TE cells, and intact human blastocysts. Using a cDNA microarray composed of 15,529 cDNAs from known and novel genes, we identify marker transcripts specific to the ICM (e.g., OCT4/POU5F1, NANOG, HMGB1, and DPPA5) and TE (e.g., CDX2, ATP1B3, SFN, and IPL), in addition to novel ICM- and TE-specific expressed sequence tags. The expression patterns suggest that the emergence of pluripotent ICM and TE cell lineages from the morula is controlled by metabolic and signaling pathways, which include inter alia, WNT, mitogen-activated protein kinase, transforming growth factor-beta, NOTCH, integrin-mediated cell adhesion, phosphatidylinositol 3-kinase, and apoptosis. These data enhance our understanding of the first step in human cellular differentiation and, hence, the derivation of both embryonic stem cells and trophoblastic stem cells from these lineages.
Free Keywords:Preimplantation development; Blastocyst; Inner cell mass; Trophectoderm; Pluripotency; Embryonic stem cells; Trophoblastic stem cells; OCT4; CDX2; Differentiation; Microarrays
Comment of the Author/Creator:E-mail: adjaye@molgen.mpg.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Reproduction and Early Development Research Group, Department of Obstetrics and Gynaecology, University of Leeds, Leeds, W. Yorkshire, United Kingdom;
Center for Reproductive Medicine & Infertility, Weill Medical College, Cornell University, New York, New York, USA
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