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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen mpi-cbg 2003     Display Documents



ID: 26951.0, MPI für molekulare Zellbiologie und Genetik / Publikationen mpi-cbg 2003
Codon-improved Cre recombinase (iCre) expression in the mouse
Authors:Shimshek, D. R.; Kim, J.; Hübner, M. R.; Spergel, D. J.; Buchholz, Frank; Casanova, E.; Stewart, A. Francis; Seeburg, P. H.; Sprengel, R.
Date of Publication (YYYY-MM-DD):2002
Title of Journal:Genesis
Volume:32
Issue / Number:1
Start Page:19
End Page:26
Review Status:not specified
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:By applying the mammalian codon usage to Cre recombinase, we improved Cre expression, as determined by immunoblot and functional analysis, in three different mammalian cell lines. The improved Cre (iCre) gene was also designed to reduce the high CpG content of the prokaryotic coding sequence, thereby reducing the chances of epigenetic silencing in mammals. Transgenic iCre expressing mice were obtained with good frequency, and in these mice loxP-mediated DNA recombination was observed in all cells expressing iCre. Moreover, iCre fused to two estrogen receptor hormone binding domains for temporal control of Cre activity could also be expressed in transgenic mice. However, Cre induction after administration of tamoxifen yielded only low Cre activity. Thus, whereas efficient activation of Cre fusion proteins in the brain needs further improvements, our studies indicate that iCre should facilitate genetic experiments in the mouse.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:222
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