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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 273092.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
How to improve quality assurance in fluorometry : fluorescence-inherent sources of error and suited fluorescence standards
Authors:Resch-Genger, U.; Hoffmann, K.; Nietfeld, W.; Engel, A.; Neukammer, J.; Nitschke, R.; Ebert, P.; Macdonald, R.
Date of Publication (YYYY-MM-DD):2005-05
Title of Journal:Journal of Fluorescence
Journal Abbrev.:J Fluorescence
Issue / Number:3
Start Page:337
End Page:362
Copyright:© Springer. Part of Springer Science+Business Media
Review Status:not specified
Audience:Experts Only
Abstract / Description:The scope of this paper is to illustrate the need for an improved quality assurance in fluorometry. For this purpose, instrumental sources of error and their influences on the reliability and comparability of fluorescence data are highlighted for frequently used photoluminescence techniques ranging from conventional macro- and microfluorometry over fluorescence microscopy and flow cytometry to microarray technology as well as in vivo fluorescence imaging. Particularly, the need for and requirements on fluorescence standards for the characterization and performance validation of fluorescence instruments, to enhance the comparability of fluorescence data, and to enable quantitative fluorescence analysis are discussed. Special emphasis is dedicated to spectral fluorescence standards and fluorescence intensity standards.
Free Keywords:Fluorescence; standard; calibration; microarray; in vivo imaging; flow cytometry
Comment of the Author/Creator:Email: ute.resch@bam.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Federal Institute for Materials Research and Testing, Working Group Optical Spectroscopy, Division I.3, BAM, Berlin, Germany;
Schott AG, Mainz, Germany;
Physikalisch-Technische Bundesanstalt, Dept. 8.3, PTB, Berlin, Germany;
Life Imaging Center, Institute of Biology I, Developmental Biology, Freiburg, Germany
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