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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 273184.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Genomewide production of multipurpose alleles for the functional analysis of the mouse genome
Authors:Schnütgen, Frank; De-Zolt, Silke; Van Sloun, Petra; Hollatz, Melanie; Floss, Thomas; Hansen, Jens; Altschmied, Joachim; Seisenberger, Claudia; Ghyselinck, Norbert B.; Ruiz, Patricia; Chambon, Pierre; Wurst, Wolfgang; von Melchner, Harald
Language:English
Date of Publication (YYYY-MM-DD):2005-05-03
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America (Washington, DC)
Journal Abbrev.:Proc Natl Acad Sci U S A
Volume:102
Issue / Number:20
Start Page:7221
End Page:7226
Copyright:© 2005 by the National Academy of Sciences
Review Status:not specified
Audience:Experts Only
Abstract / Description:A type of retroviral gene trap vectors has been developed that can induce conditional mutations in most genes expressed in mouse embryonic stem (ES) cells. The vectors rely on directional site-specific recombination systems that can repair and reinduce gene trap mutations when activated in succession. After the gene traps are inserted into the mouse genome, genetic mutations can be produced at a particular time and place in somatic cells. In addition to their conditional features, the vectors create multipurpose alleles amenable to a wide range of postinsertional modifications. Here we have used these directional recombination vectors to assemble the largest library of ES cell lines with conditional mutations in single genes yet assembled, presently totaling 1,000 unique genes. The trapped ES cell lines, which can be ordered from the German Gene Trap Consortium, are freely available to the scientific community.
Free Keywords:conditional mutagenesis; ES cells; gene trapping; site-specific recombination; insertional mutagenesis
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Department of Molecular Hematology, University of Frankfurt Medical School, 60590 Frankfurt am Main, Germany;
Institute of Developmental Genetics, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany;
Institut Clinique de la Souris and Institut de Génétique et de Biologie Moléculaire et Cellulaire, CU de Strasbourg, 67404 Illkirch Cedex, France
Identifiers:ISSN:0027-8424
DOI:10.1073/pnas.0502273102
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