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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biomedizinische Optik     Display Documents



ID: 273206.0, MPI für medizinische Forschung / Abteilung Biomedizinische Optik
The recombination activation gene 1 (Rag1) is expressed in a subset of zebrafish olfactory neurons but is not required for axon targeting or amino acid detection
Translation of Title:The recombination activation gene 1 (Rag1) is expressed in a subset of zebrafish olfactory neurons but is not required for axon targeting or amino acid detection
Authors:Feng, Bo; Bulchand, Sarada; Yaksi, Emre; Friedrich, Rainer W.; Jesuthasan, Suresh
Language:English
Date of Publication (YYYY-MM-DD):2005-07-05
Title of Journal:BMC Neuroscience
Journal Abbrev.:BMC Neuroscience
Volume:6
Issue / Number:46
Start Page:1
End Page:12
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Rag1 (Recombination activation gene−1) mediates genomic rearrangement and is essential for adaptive immunity in vertebrates. This gene is also expressed in the olfactory epithelium, but its function there is unknown. Using a transgenic zebrafish line and immunofluorescence, we show that Rag1 is expressed and translated in a subset of olfactory sensory neurons (OSNs). Neurons expressing GFP under the Rag1 promoter project their axons to the lateral region of the olfactory bulb only, and axons with the highest levels of GFP terminate in a single glomerular structure. A subset of GFP−expressing neurons contain Gαo, a marker for microvillous neurons. None of the GFP−positive neurons express Gαolf, Gαq or the olfactory marker protein OMP. Depletion of RAG1, by morpholino−mediated knockdown or mutation, did not affect axon targeting. Calcium imaging indicates that amino acids evoke chemotopically organized glomerular activity patterns in a Rag1 mutant. Rag1 expression is restricted to a subpopulation of zebrafish olfactory neurons projecting to the lateral olfactory bulb. RAG1 catalytic activity is not essential for axon targeting, nor is it likely to be required for regulation of odorant receptor expression or the response of OSNs to amino acids.
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomedizinische Optik
Relations:Has References-URI:http://www.biomedcentral.com/sfx_links.asp?ui=1471-2202-6-46&bibl=B43
Has References-URI:http://www.biomedcentral.com/sfx_links.asp?ui=1471-2202-6-46&bibl=B42
Has References-URI:http://www.biomedcentral.com/sfx_links.asp?ui=1471-2202-6-46&bibl=B41
Has References-URI:http://www.biomedcentral.com/sfx_links.asp?ui=1471-2202-6-46&bibl=B40
Has References-URI:http://www.biomedcentral.com/sfx_links.asp?ui=1471-2202-6-46&bibl=B39
Identifiers:LOCALID:6508
URI:http://www.biomedcentral.com/1471-2202/6/46
URI:http://www.biomedcentral.com/1471-2202/6/46
URI:http://www.biomedcentral.com/1471-2202/6/46/abstra...
DOI:10.1186/1471-2202-6-46
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