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          Institute: MPI für Infektionsbiologie     Collection: Department of Immunology     Display Documents



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ID: 273571.0, MPI für Infektionsbiologie / Department of Immunology
Disruption of Toxoplasma gondii Parasitophorous Vacuoles by the Mouse p47-Resistance GTPases
Authors:Martens, Sascha; Parvanova, Iana; Zerrahn, Jens; Griffiths, Gareth; Schell, Gudrun; Reichmann, Gaby; Howard, Jonathan C.
Language:English
Date of Publication (YYYY-MM-DD):2005
Title of Journal:PLoS Pathogens
Volume:1
Issue / Number:3
Sequence Number of Article:e24
Copyright:© 2005 Martens et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The p47 GTPases are essential for interferon-γ-induced cell-autonomous immunity against the protozoan parasite, Toxoplasma gondii, in mice, but the mechanism of resistance is poorly understood. We show that the p47 GTPases, including IIGP1, accumulate at vacuoles containing T. gondii. The accumulation is GTP-dependent and requires live parasites. Vacuolar IIGP1 accumulations undergo a maturation-like process accompanied by vesiculation of the parasitophorous vacuole membrane. This culminates in disruption of the parasitophorous vacuole and finally of the parasite itself. Over-expression of IIGP1 leads to accelerated vacuolar disruption whereas a dominant negative form of IIGP1 interferes with interferon-γ-mediated killing of intracellular parasites. Targeted deletion of the IIGP1 gene results in partial loss of the IFN-γ-mediated T. gondii growth restriction in mouse astrocytes.
External Publication Status:published
Document Type:Article
Communicated by:Hilmar Fünning
Affiliations:MPI für Infektionsbiologie/Department of Immunology
External Affiliations:Institute for Genetics, University of Cologne, Cologne, Germany
European Molecular Biology Laboratory, Heidelberg, Germany Institute for Medical Microbiology, Heinrich Heine University Duesseldorf, Duesseldorf, Germany
Identifiers:DOI:10.1371/journal.ppat.0010024
URL:http://pathogens.plosjournals.org/perlserv/?reques...
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