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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 275452.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Impact of mouse strain differences in innate hindlimb collateral vasculature
Authors:Helisch, A.; Wagner, S.; Khan, N.; Drinane, M.; Wolfram, S.; Heil, M.; Ziegelhoeffer, T.; Brandt, U.; Pearlman, J. D.; Swartz, H. M.; Schaper, W.
Date of Publication (YYYY-MM-DD):2006-03
Title of Journal:Arterioscler Thromb Vasc Biol
Journal Abbrev.:Arterioscler Thromb Vasc Biol
Issue / Number:3
Start Page:520
End Page:526
Audience:Not Specified
Abstract / Description:OBJECTIVE: To assess the importance of genetic background for collateral artery development. METHODS AND RESULTS: C57BL/6, BALB/c and 129S2/Sv mice were studied after femoral artery ligation by laser Doppler imaging, visible light oximetry, time-of-flight-magnetic resonance imaging, and treadmill testing; C57BL/6 and BALB/c also underwent electron paramagnetic resonance (EPR) oximetry, x-ray angiography, and histology. C57BL/6 had the least initial distal ischemia and most complete recovery. BALB/c had the most severe initial ischemia and poorest recovery. BALB/c had some vasodilatory reserve in their ligated limbs not seen in the other strains at 3 weeks. By in vivo TOF-magnetic resonance angiography, C57BL/6 had larger preexistent and developed collaterals. By x-ray angiography, C57BL/6 had a higher collateral-dependent filling score and number of visible collaterals immediately after femoral ligation and a higher number of visible collaterals at 1 week but not at 4 weeks. EPR oximetry and histology revealed hypoxia and tissue damage in regions of collateral growth of BALB/c but not C57BL/6 mice. In C57BL/6 BrdUrd uptake in the thigh was limited to larger vessels and isolated perivascular cells. Proliferative activity in collateral arterioles was similar in both strains. CONCLUSIONS: Genetic differences in preexistent collateral vasculature can profoundly affect outcome and milieu for compensatory collateral artery growth after femoral artery occlusion.
Free Keywords:Animals Comparative Study *Disease Models, Animal Electron Spin Resonance Spectroscopy Femoral Artery Hindlimb/blood supply Hyperemia/genetics/pathology/physiopathology Ischemia/*genetics/pathology/physiopathology Ligation Magnetic Resonance Angiography Male Mice *Mice, Inbred BALB C *Mice, Inbred C57BL Muscle, Skeletal/blood supply/pathology Neovascularization, Physiologic/*genetics Organ Size Oximetry Oxyhemoglobins/metabolism Research Support, Non-U.S. Gov't Species Specificity
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
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