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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 275687.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
A human protein-protein interaction network : a resource for annotating the proteome
Authors:Stelzl, Ulrich; Worm, Uwe; Lalowski, Maciej; Haenig, Christian; Brembeck, Felix H.; Goehler, Heike; Stroedicke, Martin; Zenkner, Martina; Schoenherr, Anke; Koeppen, Susanne; Timm, Jan; Mintzlaff, Sascha; Abraham, Claudia; Bock, Nicole; Kietzmann, Silvia; Goedde, Astrid; Toksöz, Engin; Droege, Anja; Krobitsch, Sylvia; Korn, Bernhard; Birchmeier, Walter; Lehrach, Hans; Wanker, Erich E.
Language:English
Date of Publication (YYYY-MM-DD):2005-09-15
Title of Journal:Cell
Volume:122
Issue / Number:6
Start Page:957
End Page:968
Copyright:© 2006 Elsevier B.V.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Protein-protein interaction maps provide a valuable framework for a better understanding of the functional organization of the proteome. To detect interacting pairs of human proteins systematically, a protein matrix of 4456 baits and 5632 preys was screened by automated yeast two-hybrid (Y2H) interaction mating. We identified 3186 mostly novel interactions among 1705 proteins, resulting in a large, highly connected network. Independent pull-down and coimmunoprecipitation assays validated the overall quality of the Y2H interactions. Using topological and GO criteria, a scoring system was developed to define 911 high-confidence interactions among 401 proteins. Furthermore, the network was searched for interactions linking uncharacterized gene products and human disease proteins to regulatory cellular pathways. Two novel Axin-1 interactions were validated experimentally, characterizing ANP32A and CRMP1 as modulators of Wnt signaling. Systematic human protein interaction screens can lead to a more comprehensive understanding of protein function and cellular processes.
Comment of the Author/Creator:Email: erich.w@mdc-berlin.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Max Delbrueck Center for Molecular Medicine, 13092 Berlin-Buch, Germany;
German Genome Resource Center, 69120 Heidelberg, Germany
Identifiers:ISSN:0092-8674
DOI:10.1016/j.cell.2005.08.029
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