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          Institute: MPI für Polymerforschung     Collection: MPI Polymerforschung     Display Documents

ID: 28351.0, MPI für Polymerforschung / MPI Polymerforschung
No influence of magnetic fields on cell cycle progression using conditions relevant for patients during MRI
Authors:Schiffer, I. B.; Schreiber, W. G.; Graf, Robert; Schreiber, E. M.; Jung, D.; Rose, D. M.; Hehn, M.; Gebhard, S.; Sagemüller, J.; Spiess, Hans Wolfgang; Oesch, F.; Thelen, M.; Hengstler, J. G.
Date of Publication (YYYY-MM-DD):2003-05
Title of Journal:Bioelectromagnetics
Journal Abbrev.:Bioelectromagnetics
Issue / Number:4
Start Page:241
End Page:250
Review Status:not specified
Audience:Not Specified
Abstract / Description:The purpose of this study was to examine whether exposure to magnetic fields (MFs) relevant for magnetic resonance imaging (MRI) in clinical routine influences cell cycle progression in two tumor cell lines in vitro. HL60 and EA2 cells were exposed to four types of MFs: (i) static MF of 1.5 and 7.05 T, (ii) extremely low frequency magnetic gradient fields (ELFMGFs) with ± 10 mT/m and 100 Hz, as well as ± 100 mT/m and 100 Hz, (iii) pulsed high frequency MF in the radiofrequency (RF) range (63.6 MHz, 5.8 μT), and (iv) a combination of (i-iii). Exposure periods ranged from 1 to 24 h. Cell cycle distribution (G0/G1, S, and G2/M phases) was analyzed by flow cytometry. Cell cycle analysis did not reveal differences between the exposed and the control cells. As expected, positive controls with irradiated (8 Gy) HL60 and EA2 cells showed a strong G2/M arrest. Using conditions that are relevant for patients during MRI, no influence of MFs on cell cycle progression was observed in these cell lines. Care was taken to control secondary parameters of influence, such as vibration by the MR scanner or temperature to avoid false positive results.
Free Keywords:static magnetic field; pulsed bipolar magnetic gradient; ELF gradient magnetic field; pulsed high frequency electromagnetic field; FACS; cell cycle
Comment of the Author/Creator:Date: 2003, MAY
External Publication Status:published
Document Type:Article
Affiliations:MPI für Polymerforschung
External Affiliations:Univ Mainz, Inst Toxicol, Obere Zahlbacherstr 67, D-55131 Mainz, Germany; Univ Mainz, Dept Radiol, D-6500 Mainz, Germany; Univ Mainz, Dept Radiat Therapy, D-6500 Mainz, Germany; Univ Mainz, Inst Occupat Med, D-6500 Mainz, Germany
Identifiers:ISI:000182526400003 [ID No:1]
ISSN:0197-8462 [ID No:2]
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