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          Institute: MPI für Polymerforschung     Collection: MPI Polymerforschung     Display Documents



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ID: 28387.0, MPI für Polymerforschung / MPI Polymerforschung
Fusion of small unilamellar vesicles onto laterally mixed self- assembled monolayers of thiolipopeptides
Authors:Baumgart, T.; Kreiter, Maximilian; Lauer, H.; Naumann, Renate; Jung, G.; Jonczyk, A.; Offenhäusser, Andreas; Knoll, Wolfgang
Language:English
Date of Publication (YYYY-MM-DD):2003-02-15
Title of Journal:Journal of Colloid and Interface Science
Journal Abbrev.:J. Colloid Interface Sci.
Volume:258
Issue / Number:2
Start Page:298
End Page:309
Review Status:not specified
Audience:Not Specified
Abstract / Description:Monolayers of the thiolipopeptide NH2-Cys-Ala-Ser-Ala-Ala-Ser- Ser-Ala-Pro-Ser-Ser-(Myr)Lys(Myr)-OH (III) were formed on gold surfaces by self-assembly, mixed with a lateral spacer of the same peptide composition, NH2-Cys-Ala-Ser-Ala-Ala-Ser-Ser- AlaPro-Ser-Ser-Lys-OH (I). Different mixing ratios were employed ranging from 0.1 to 1, corresponding to 10-100% thiolipopeptide. These self-assembled monolayers (SAMs) were then exposed to a suspension of liposomes with the aim of forming lipid bilayers as a function of the mixing ratio. A clear optimum with respect to homogeneity and electrical properties of the membranes was obtained in the middle region (0.5) of mixing ratio, as revealed by surface plasmon resonance spectroscopy, impedance spectroscopy, and fluorescence microscopy. The combination of these methods was shown to be a powerful tool, although a true lipid bilayer was not obtained. Instead, vesicle adsorption was shown to be the predominant process, and FRAP (fluorescence recovery after photobleaching) measurements showed that the films were not fluid on the micrometer length scale. (C) 2003 Elsevier Science (USA). All rights reserved.
Free Keywords:solid-supported lipid bilayers; solid-supported lipid membranes; tethered membranes; thiolipopeptide; thiopeptide; surface plasmon resonance spectroscopy; impedance spectroscopy; fluorescence microscopy
Comment of the Author/Creator:Date: 2003, FEB 15
External Publication Status:published
Document Type:Article
Affiliations:MPI für Polymerforschung
External Affiliations:Univ Tübingen, D-72076 Tübingen, Germany; Merck KGaA, D-64271 Darmstadt, Germany
Identifiers:ISI:000181558900012 [ID No:1]
ISSN:0021-9797 [ID No:2]
LOCALID:P-03-17
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