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          Institute: MPI für Polymerforschung     Collection: MPI Polymerforschung     Display Documents



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ID: 28569.0, MPI für Polymerforschung / MPI Polymerforschung
PDMS device for patterned application of microfluids to neuronal cells arranged by microcontact printing
Authors:Thiebaud, P.; Lauer, L.; Knoll, Wolfgang; Offenhäusser, Andreas
Language:English
Date of Publication (YYYY-MM-DD):2002-01
Title of Journal:Biosensors & Bioelectronics
Journal Abbrev.:Biosens. Bioelectron.
Volume:17
Issue / Number:1-2
Start Page:87
End Page:93
Review Status:No review
Audience:Not Specified
Abstract / Description:A microfluidic device in polydimethylsiloxane (PDMS) consisting of an eight lines micro-injection array integrated in a base flow channel has been realized. The device is assembled from multiple PDMS parts, which have been moulded using notably micromachined masters in SU-8 photoresist. In contact with a planar substrate., up to eight independent laminar flow lines with cross-sections of 100 x 200 mum(2) can be generated. Dedicated for the application of pharmaceutical compounds to electrogenic cells in vitro, this device was tested with a neuronal cell line, Mzl-cells. These were cultured on lines of laminin deposited onto polystyrene substrates by microcontact printing. We were able to inject into this culture multiple lines of coloured PBS in parallel to the orientation of cellular growth. No mixing between the individual flow lines did occur. (C) 2002 Elsevier Science B.V. All rights reserved.
Free Keywords:microinjector array; polydimethylsiloxane; SU-8 photoresist; microcontact printing; PCC12 Mzl neuroblastoma cells
Comment of the Author/Creator:Date: 2002, JAN
External Publication Status:published
Document Type:Article
Affiliations:MPI für Polymerforschung
External Affiliations:Res Ctr Julich, Inst Thin Films & Interfaces, D-52425 Julich, Germany
Identifiers:ISI:000173263200011 [ID No:1]
ISSN:0956-5663 [ID No:2]
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