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          Institute: MPI für Polymerforschung     Collection: MPI Polymerforschung     Display Documents

ID: 28642.0, MPI für Polymerforschung / MPI Polymerforschung
Monolayer formation of cytochrome b(562) on gold surfaces and its reconstitution reaction, studied by surface plasmon resonance spectroscopy
Authors:Kobayashi, K.; Shimizu, M.; Nagamune, T.; Sasabe, H.; Fang, Y.; Knoll, Wolfgang
Date of Publication (YYYY-MM-DD):2002-08
Title of Journal:Bulletin of the Chemical Society of Japan
Journal Abbrev.:Bull. Chem. Soc. Jpn.
Issue / Number:8
Start Page:1707
End Page:1713
Review Status:No review
Audience:Not Specified
Abstract / Description:Association and dissociation reactions of cytochrome b(562) on solid surfaces were investigated by means of surface plasmon resonance spectroscopy. Propionates of side chains in the heme molecule were modified with sulfur moiety as an anchor part to be adsorbed on the gold substrate. This modified heme was firstly reconstituted with apo-protein in solution. This reconstituted holo-protein was then adsorbed on either bare gold substrate or pre-modified gold substrate. Native cytochrome b(562) was also adsorbed to the same substrates for the control experiment. The reconstituted cytochrome b(562) was successfully adsorbed on the pre-treated gold substrate without denaturation in structure, probably with homogeneous anisotropic orientation. Guanidine hydrochloride in the buffer of pH 5.0 was applied to remove the apoprotein from the surface, with the thiolated heme derivative remaining on the surfaces. Apo-protein was again introduced to the same surface for the reconstitution of holo-cyt.b(562). The success of the association and dissociation reactions between apo-protein and tethered heme molecules on the surfaces was confirmed by the: optical thickness changes.
Comment of the Author/Creator:Date: 2002, AUG
External Publication Status:published
Document Type:Article
Affiliations:MPI für Polymerforschung
External Affiliations:Univ Tokyo, Sch Engn, Dept Chem & Biotechnol, Bunkyo Ku, 7-3-1 Hongo, Tokyo 1138656, Japan; RIKEN, Inst Phys & Chem Res, Frontier Res Program, Wako, Saitama 3510198, Japan
Identifiers:ISI:000177602600006 [ID No:1]
ISSN:0009-2673 [ID No:2]
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