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          Institute: MPI für Pflanzenzüchtungsforschung     Collection: Dept. Plant Microbe Interactions (Paul Schulze-Lefert)     Display Documents

ID: 28724.0, MPI für Pflanzenzüchtungsforschung / Dept. Plant Microbe Interactions (Paul Schulze-Lefert)
Barley disease resistance gene analogs of the NBS-LRR class: identification and mapping
Authors:Madsen, L. H.; Collins, N. C.; Rakwalska, M.; Backes, G.; Sandal, N.; Krusell, L.; Jensen, J.; Waterman, E. H.; Jahoor, A.; Ayliffe, M.; Pryor, A. J.; Langridge, P.; Schulze-Lefert, P.; Stougaard, J.
Date of Publication (YYYY-MM-DD):2003-04
Title of Journal:Molecular Genetics and Genomics
Journal Abbrev.:Mol. Genet. Genomics
Issue / Number:1
Start Page:150
End Page:161
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The majority of verified plant disease resistance genes isolated to date are of the NBS-LRR class, encoding proteins with a predicted nucleotide binding site (NBS) and a leucine- rich repeat (LRR) region. We took advantage of the sequence conservation in the NBS motif to clone, by PCR, gene fragments from barley representing putative disease resistance genes of this class. Over 30 different resistance gene analogs (RGAs) were isolated from the barley cultivar Regatta. These were grouped into 13 classes based on DNA sequence similarity. Actively transcribed genes were identified from all classes but one, and cDNA clones were isolated to derive the complete NBS- LRR protein sequences. Some of the NBS-LRR genes exhibited variation with respect to whether and where particular introns were spliced, as well as frequent premature polyadenylation. DNA sequences related to the majority of the barley RGAs were identified in the recently expanded public rice genomic sequence database, indicating that. the rice sequence can be used to extract a large proportion of the RGAs from barley and other cereals. Using a combination of RFLP and PCR marker techniques, representatives of all barley RGA gene classes were mapped in the barley genome, to all chromosomes except 4H. A number of the RGA loci map in the vicinity of known disease resistance loci, and the association between RGA S-120 and the nematode resistance locus Ha2on chromosome 2H was further tested by co-segregation analysis. Most of the RGA sequences reported here have not been described previously, and represent a useful resource as candidates or molecular markers for disease resistance genes in barley and other cereals. Electronic Supplementary Material Supplementary material is available for this article if you access the article at http://dx.doi.org/10.1007/s00438-003-0823-5. A link in the frame on the left on that,page takes you directly to the supplementary material.
Free Keywords:barley; disease resistance gene analogs (RGAs); NBS-LRR proteins; mapping
Comment of the Author/Creator:Date: 2003, APR
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Pflanzenzüchtungsforschung
External Affiliations:Aarhus Univ, Dept Mol Biol, Gustav Wieds Vej 10, DK-8000 Aarhus; C, Denmark; Aarhus Univ, Dept Mol Biol, DK-8000 Aarhus C, Denmark; John Innes Ctr Plant Sci Res, Sainsbury Lab, Norwich NR4 7UH, Norfolk, England; Riso Natl Lab, Plant Res Dept, DK-4000 Roskilde, Denmark; CSIRO, Plant Ind, Canberra, ACT 2601, Australia; Univ Adelaide, Dept Plant Sci, Fac Sci, Glen Osmond, SA 5064, Australia; Max Planck Inst Plant Breeding Res, Dept Plant Microbe Interact, D-50829 Cologne, Germany
Identifiers:ISI:000183032400016 [ID No:1]
ISSN:1617-4615 [ID No:2]
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