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          Institute: MPI für Pflanzenzüchtungsforschung     Collection: Dept. Plant Developmental Biology (George Coupland)     Display Documents



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ID: 28777.0, MPI für Pflanzenzüchtungsforschung / Dept. Plant Developmental Biology (George Coupland)
A polyamine metabolon involving aminopropyl transferase complexes in Arabidopsis
Authors:Panicot, M.; Minguet, E. G.; Ferrando, A.; Alcázar, R.; Blazquez, M. A.; Carbonell, J.; Altabella, T.; Koncz, C.; Tiburcio, A. F.
Language:English
Date of Publication (YYYY-MM-DD):2002-10
Title of Journal:Plant Cell
Journal Abbrev.:Plant Cell
Volume:14
Issue / Number:10
Start Page:2539
End Page:2551
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The conversion of putrescine to spermidine in the biosynthetic pathway of plant polyamines is catalyzed by two closely related spermidine synthases, SPDS1 and SPDS2, in Arabidopsis. In the yeast two-hybrid system, SPDS2 was found to interact with SPDS1 and a novel protein, SPMS (spermine synthase), which is homologous with SPDS2 and SPDS1. SPMS interacts with both SPDS1 and SPDS2 in yeast and in vitro. Unlike SPDS1 and SPDS2, SPMS failed to suppress the speDelta3 deficiency of spermidine synthase in yeast. However, SPMS was able to complement the speDelta4 spermine deficiency in yeast, indicating that SPMS is a novel spermine synthase. The SPDS and SPMS proteins showed no homodimerization but formed heterodimers in vitro. Pairwise coexpression of hemagglutinin- and c-Myc epitope-labeled proteins in Arabidopsis cells confirmed the existence of coimmunoprecipitating SPDS1-SPDS2 and SDPS2-SPMS heterodimers in vivo. The epitope-labeled SPDS and SPMS proteins copurified with protein complexes ranging in size from 650 to 750 kD. Our data demonstrate the existence of a metabolon involving at least the last two steps of polyamine biosynthesis in Arabidopsis.
Comment of the Author/Creator:Date: 2002, OCT
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Pflanzenzüchtungsforschung
External Affiliations:Univ Barcelona, Fac Farm, Unitat Fisiol Vegetal, Diagonal 643,; E-08028 Barcelona, Spain; Univ Barcelona, Fac Farm, Unitat Fisiol Vegetal, E-08028 Barcelona, Spain; Univ Politecn Valencia, CSIC, Inst Biol Mol & Celular Plantas, Valencia 46022, Spain; Max Planck Inst Zuchtungsforsch, D-50829 Cologne, Germany
Identifiers:ISI:000178740100017 [ID No:1]
ISSN:1040-4651 [ID No:2]
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