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          Institute: MPI für Pflanzenzüchtungsforschung     Collection: Dept. Plant Developmental Biology (George Coupland)     Display Documents



ID: 28786.0, MPI für Pflanzenzüchtungsforschung / Dept. Plant Developmental Biology (George Coupland)
Antagonistic regulation of flowering-time gene SOC1 by CONSTANS and FLC via separate promoter motifs
Authors:Hepworth, S. R.; Valverde, F.; Ravenscroft, D.; Mouradov, A.; Coupland, G.
Language:English
Date of Publication (YYYY-MM-DD):2002-08-15
Title of Journal:EMBO Journal
Journal Abbrev.:Embo J.
Volume:21
Issue / Number:16
Start Page:4327
End Page:4337
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Flowering in Arabidopsis is controlled by endogenous and environmental signals relayed by distinct genetic pathways. The MADS-box flowering-time gene SOC1 is regulated by several pathways and is proposed to co-ordinate responses to environmental signals. SOC1 is directly activated by CONSTANS (CO) in long photoperiods and is repressed by FLC, a component of the vernalization (low-temperature) pathway. We show that in transgenic plants overexpressing CO and FLC, these proteins regulate flowering time antagonistically and FLC blocks transcriptional activation of SOC1 by CO. A series of SOC1::GUS reporter genes identified a 351 bp promoter sequence that mediates activation by CO and repression by FLC. A CArG box (MADS-domain protein binding element) within this sequence was recognized specifically by FLC in vitro and mediated repression by FLC in vivo, suggesting that FLC binds directly to the SOC1 promoter. We propose that CO is recruited to a separate promoter element by a DNA-binding factor and that activation by CO is impaired when FLC is bound to an adjacent CArG motif.
Free Keywords:Arabidopis; CONSTANS; FLC; flowering; SOC1
Comment of the Author/Creator:Date: 2002, AUG 15
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Pflanzenzüchtungsforschung
External Affiliations:John Innes Ctr Plant Sci Res, Colney Lane, Norwich NR4 7UH,; Norfolk, England; John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk, England; Max Planck Inst Zuchtungsforsch, D-50829 Cologne, Germany
Identifiers:ISI:000177355300013 [ID No:1]
ISSN:0261-4189 [ID No:2]
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