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          Institute: MPI für Pflanzenzüchtungsforschung     Collection: Dept. Plant Developmental Biology (George Coupland)     Display Documents



ID: 28790.0, MPI für Pflanzenzüchtungsforschung / Dept. Plant Developmental Biology (George Coupland)
Knock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency accompanied by cell differentiation defects
Authors:Henriques, R.; Jasik, J.; Klein, M.; Martinoia, E.; Feller, U.; Schell, J.; Pais, M. S.; Koncz, C.
Language:English
Date of Publication (YYYY-MM-DD):2002-11
Title of Journal:Plant Molecular Biology
Journal Abbrev.:Plant Mol.Biol.
Volume:50
Issue / Number:4
Start Page:587
End Page:597
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:IRT1 and IRT2 are members of the Arabidopsis ZIP metal transporter family that are specifically induced by iron deprivation in roots and act as heterologous suppressors of yeast mutations inhibiting iron and zinc uptake. Although IRT1 and IRT2 are thought to perform redundant functions as root- specific metal transporters, insertional inactivation of the IRT1 gene alone results in typical symptoms of iron deficiency causing severe leaf chlorosis and lethality in soil. The irt1 mutation is characterized by specific developmental defects, including a drastic reduction of chloroplast thylakoid stacking into grana and lack of palisade parenchyma differentiation in leaves, reduced number of vascular bundles in stems, and irregular patterns of enlarged endodermal and cortex cells in roots. Pulse labeling with (5)9Fe through the root system shows that the irt1 mutation reduces iron accumulation in the shoots. Short-term labeling with (6)5Zn reveals no alteration in spatial distribution of zinc, but indicates a lower level of zinc accumulation. In comparison to wild-type, the irt1 mutant responds to iron and zinc deprivation by altered expression of certain zinc and iron transporter genes, which results in the activation of ZIP1 in shoots, reduction of ZIP2 transcript levels in roots, and enhanced expression of IRT2 in roots. These data support the conclusion that IRT1 is an essential metal transporter required for proper development and regulation of iron and zinc homeostasis in Arabidopsis.
Free Keywords:Arabidopsis; insertion mutagenesis; IRT; metal-transporter; ZIP
Comment of the Author/Creator:Date: 2002, NOV
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Pflanzenzüchtungsforschung
External Affiliations:Max Planck Inst Zuchtungsforsch, Carl Von Linne Weg 10, D-50829; Cologne, Germany; Max Planck Inst Zuchtungsforsch, D-50829 Cologne, Germany; Univ Lisbon, ICAT, P-1749016 Lisbon, Portugal; Comenius Univ, Dept Plant Physiol, Bratislava 84215, Slovakia; Univ Neuchatel, Inst Bot, Lab Physiol Vegetale, CH-2007 Neuchatel, Switzerland; Univ Bern, Inst Plant Sci, CH-3013 Bern, Switzerland
Identifiers:ISI:000177679200001 [ID No:1]
ISSN:0167-4412 [ID No:2]
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