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          Institute: MPI für Pflanzenzüchtungsforschung     Collection: Dept. Plant Microbe Interactions (Paul Schulze-Lefert)     Display Documents

ID: 28807.0, MPI für Pflanzenzüchtungsforschung / Dept. Plant Microbe Interactions (Paul Schulze-Lefert)
NHL25 and NHL3, two NDR1/HIN1-like genes in Arabidopsis thaliana with potential role(s) in plant defense
Authors:Varet, A.; Parker, J.; Tornero, P.; Nass, N.; Nuernberger, T.; Dangl, J. L.; Scheel, D.; Lee, J.
Date of Publication (YYYY-MM-DD):2002-06
Title of Journal:Molecular Plant-Microbe Interactions
Journal Abbrev.:Mol. Plant-Microbe Interact.
Issue / Number:6
Start Page:608
End Page:616
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The Arabidopsis genome contains 28 genes with sequence homology to the Arabidopsis NDR1 gene and the tobacco HIN1 gene. Expression analysis of eight of these genes identified two (NHL25 and VHL3 for NDR1/HIN1-like) that show pathogen- dependent mRNA accumulation. Transcripts did not accumulate during infection with virulent Pseudomonas syringae pv. tomato DC3000 but did accumulate specifically when the bacteria carried any of the four avirulence genes avrRpm1, avrRpt2, avrB, or avrRps4. Furthermore, expression of avrRpt2 in plants containing the corresponding resistance gene, RPS2, was sufficient to induce transcript accumulation. However, during infection with an avirulent oomycete, Peronospora parasitica isolate Cala-2, only NHL25 expression was reproducibly induced. Salicylic acid (SA) treatment can induce expression of NHL25 and NHL3. Studies performed on nahG plants showed that, during interaction with avirulent bacteria, only the expression of NHL25 but not that of NHL3 was affected. This suggests involvement of separate SA-dependent and SA-independent pathways, respectively, in the transcriptional activation of these genes. Bacteria-induced gene expression was not abolished in ethylene- (etr1-3 and ein2-1) and jasmonate- (coil-1) insensitive mutants or in mutants impaired in disease resistance (ndr1-1 and pad4-1). Interestingly, NHL3 transcripts accumulated after infiltration with the avirulent hrcC mutant of Pseudomonas syringae pv. tomato DC3000 and nonhost bacteria but not with the virulent Pseudomonas syringae pv. tomato DC3000, suggesting that virulent bacteria may suppress NHL3 expression during pathogenesis. Hence, the expression patterns and sequence homology to NDR1 and HIN1 suggest one or more potential roles for these genes in plant resistance.
Free Keywords:gene-for-gene; wounding
Comment of the Author/Creator:Date: 2002, JUN
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Pflanzenzüchtungsforschung
External Affiliations:Inst Plant Biochem, Dept Stress & Dev Biol, Weinberg 3, D-06120; Halle Saale, Germany; Inst Plant Biochem, Dept Stress & Dev Biol, D-06120 Halle Saale, Germany; Max Planck Inst Plant Breeding Res, Dept Mol Phytopathol, D-50829 Cologne, Germany; Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA; Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC 27599 USA; Univ N Carolina, Curriculum Genet, Chapel Hill, NC 27599 USA
Identifiers:ISI:000175938200011 [ID No:1]
ISSN:0894-0282 [ID No:2]
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