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          Institute: MPI für Pflanzenzüchtungsforschung     Collection: Dept. Molecular Plant Genetics (Heinz Saedler)     Display Documents

ID: 28812.0, MPI für Pflanzenzüchtungsforschung / Dept. Molecular Plant Genetics (Heinz Saedler)
Evolution of class B floral homeotic proteins: Obligate heterodimerization originated from homodimerization
Authors:Winter, K. U.; Weiser, C.; Kaufmann, K.; Bohne, A.; Kirchner, C.; Kanno, A.; Saedler, H.; Theissen, G.
Date of Publication (YYYY-MM-DD):2002-05
Title of Journal:Molecular Biology and Evolution
Journal Abbrev.:Mol. Biol. Evol.
Issue / Number:5
Start Page:587
End Page:596
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:The class B floral homeotic genes from the higher eudicot model systems Arabidopsis and Antirrhinum are involved in specifying the identity of petals and stamens during flower development. These genes exist in two different types termed DEF- and GLO- like genes. The proteins encoded by the class B genes are stable and functional in the cell only as heterodimeric complexes of a DEF- and a GLO-like protein. In line with this. heterodimerization is obligate for DNA binding in vitro. The genes whose products have to heterodimerize to be stable and functional are each other's closest relatives within their genomes. This suggests that the respective genes originated by gene duplication. and that heterodimerization is of relative recent origin and evolved from homodimerization. To test this hypothesis we hay e investigated the dimerization behavior of putative B proteins from phylogenetic informative taxa, employing electrophoretic mobility shift assays and the yeast two-hybrid system. We find that an ancestral B protein from the gymnosperm Gnetum gnemon birds DNA in a sequence-specific manner as a homodimer. Of the two types of B proteins from the monocot Lilium regale. the GLO-like protein is still able to homodimerize, whereas the DEF-like protein binds to DNA only as a heterodimeric complex with the GLO-like protein. These data suggest that heterodimerization evolved in two steps after a gene duplication that gave rise to DEF- and GLO-like genes. Heterodimerization may have originated after the gymnosperm- angiosperm split about 300 MYA but before the monocot-eudicot split 140-200 MYA. Heterodimerization may have become obligate for both types of flowering plant B proteins in the eudicot lineage after the monocot-eudicot split.
Free Keywords:MADS-box gene; homeotic gene; gymnosperm; angiosperm; evolution; dimerization
Comment of the Author/Creator:Date: 2002, MAY
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Pflanzenzüchtungsforschung
External Affiliations:Max Planck Inst Zuchtungsforsch, Abt Mol Pflanzengenet, Carl; von Linne Weg 10, D-50829 Cologne, Germany; Max Planck Inst Zuchtungsforsch, Abt Mol Pflanzengenet, D-50829 Cologne, Germany
Identifiers:ISI:000175605900001 [ID No:1]
ISSN:0737-4038 [ID No:2]
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