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          Institute: MPI für Evolutionsbiologie     Collection: Ecophysiology     Display Documents

ID: 291473.0, MPI für Evolutionsbiologie / Ecophysiology
"Spectral fingerprinting" for specific algal groups on sediments in situ: a new sensor
Authors:Aberle, N.; Beutler, M.; Moldaenke, C.; Witlshire, K. H.
Date of Publication (YYYY-MM-DD):2006-09
Title of Journal:Archiv für Hydrobiologie
Journal Abbrev.:Arch. Hydrobiol.
Issue / Number:1-4
Start Page:575
End Page:592
Title of Issue:"Natural selection is ecology in action". Dedicated to Professor Dr. Winfried Lampert on the occasion of his 65th birthday
Full name of Issue-Editor(s):Boersma, Maarten; Santer, Barbara
Review Status:not specified
Audience:Not Specified
Abstract / Description:Currently it is still extremely difficult to adequately sample populations of microalgae on sediments for large-scale biomass determination. We have now devised a prototype of a new benthic sensor (BenthoFluor) for the quantitative and qualitative assessment of microphytobenthos populations in situ. This sensor enables a high spatial and temporal resolution and a rapid evaluation of the community structure and distribution. These determinations are based on the concept that five spectral excitation ranges can be used to differentiate groups of microalgae, in situ, within a few seconds. In addition, because sediments contain a lot of yellow substances, which can affect the fluorescence and optical differentiation of the algae, the device was equipped with a UV-LED for yellow substances correction. The device was calibrated against HPLC with cultures and tested in the field. Our real-time approach can be used to monitor algal assemblage composition on sediments and is an ideal tool for investigations on the large-scale spatial and temporal variation of algal populations in sediments. Apart from the differentiation of algal populations, the BenthoFluor allows instantaneous monitoring of the chlorophyll concentrations and determination of which algae are responsible for this on the uppermost surface of sediments in the field and in experimental set-ups.
Free Keywords:pigments; excitation spectra; chlorophyll-a; benthic algae; fluorescence
External Publication Status:published
Document Type:Article
Affiliations:MPI für Limnologie/Abt. Ökophysiologie
External Affiliations:Leibniz Inst Marine Sci, IFM, GEOMAR, Dusternbrooker Weg 20, D-24105 Kiel, Germany; Kings College London, Randall Div Cell & Mol Biophys, London SE1 1UL, England; BBE Moldaenke, D-24119 Kronshagen, Germany; Alfred Wegener Inst Polar & Marine Res, Biol Anstalt Helgoland, D-27483 Helgoland, Germany
Relations:Is Version Of-EDOCID:298559
Identifiers:ISSN:0003-9136 [ID-No:1]
DOI:10.1127/0003-9136/2006/0167-0575 [ID-No:2]
LOCALID:2492/S 38569 [Listen-Nummer/S-Nummer]
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