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          Institute: MPI für Biochemie     Collection: Molecular Medicine (R. Fässler)     Display Documents



ID: 29175.0, MPI für Biochemie / Molecular Medicine (R. Fässler)
Integrin cytoplasmic domain-associated protein 1 alpha (ICAP-1 alpha) interacts directly with the metastasis suppressor nm23- H2, and both proteins are targeted to newly formed cell adhesion sites upon integrin engagement
Authors:Fournier, H. N.; Dupe-Manet, S.; Bouvard, D.; Lacombe, M. L.; Marie, C.; Block, M. R.; Albiges-Rizo, C.
Language:English
Date of Publication (YYYY-MM-DD):2002-06-07
Title of Journal:Journal of Biological Chemistry
Journal Abbrev.:J. Biol. Chem.
Volume:277
Issue / Number:23
Start Page:20895
End Page:20902
Review Status:Peer-review
Audience:Not Specified
Abstract / Description:Cell adhesion-dependent signaling implicates cytoplasmic proteins interacting with the intracellular tails of integrins. Among those, the integrin cytoplasmic domain-associated protein 1alpha (ICAP-1alpha) has been shown to interact specifically with the beta(1), integrin cytoplasmic domain. Although it is likely that this protein plays an important role in controlling cell adhesion and migration, little is known about its actual function. To search for potential ICAP-1alpha-binding proteins, we used a yeast two-hybrid screen and identified the human metastatic suppressor protein nm23-H2 as a new partner of ICAP- 1alpha. This direct interaction was confirmed in vitro, using purified recombinant ICA-P1alpha and nm23-H2, and by coimmunoprecipitation from CHO cell lysates over-expressing ICAP-1alpha. The physiological relevance of this interaction is provided by confocal fluorescence microscopy, which shows that ICAP-1alpha and nm23-H2 are colocalized in lamellipodia during the early stages of cell spreading. These adhesion sites are enriched in occupied 13, integrins and precede the formation of focal adhesions devoid of ICAP-1alpha and nm23-H2, indicating the dynamic segregation of components of matrix adhesions. This peripheral staining of ICAP-1alpha and nm23-1-12 is only observed in cells spreading on fibronectin and collagen and is absent in cells spreading on poly-L-lysine, vitronectin, or laminin. This is consistent with the fact that targeting of both ICAP-1alpha and nm23-H2 to the cell periphery is dependent on 13, integrin engagement rather than being a consequence of cell adhesion. This finding represents the first evidence that the tumor suppressor nm23-H2 could act on beta(1) integrin- mediated cell adhesion by interacting with one of the integrin partners, ICAP-1alpha.
Comment of the Author/Creator:Date: 2002, JUN 7
External Publication Status:published
Document Type:Article
Communicated by:N.N.
Affiliations:MPI für Biochemie/Molecular Medicine (R. Fässler)
External Affiliations:UJF, Fac Med Grenoble,UMR CNRS 5538, Inst Albert Bonniot, Lab; Etud Differenciat & Adherence Cellulaires, Domaine Merci, F-; 38706 La Tronche, France; UJF, Fac Med Grenoble,UMR CNRS 5538, Inst Albert Bonniot, Lab Etud Differenciat & Adherence Cellulaires, F-38706 La Tronche, France; Univ Paris 06, INSERM U402, F-75012 Paris, France
Identifiers:ISI:000176204500102 [ID No:1]
ISSN:0021-9258 [ID No:2]
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