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          Institute: MPI für molekulare Genetik     Collection: Ribosome Research Groups     Display Documents



ID: 305641.0, MPI für molekulare Genetik / Ribosome Research Groups
The E-site story: the importance of maintaining two tRNAs on the ribosome during protein synthesis
Authors:Wilson, Daniel N.; Nierhaus, Knud
Language:English
Date of Publication (YYYY-MM-DD):2006-12-01
Title of Journal:Cellular and Molecular Life Sciences
Journal Abbrev.:CMLS
Volume:63
Issue / Number:23
Start Page:2725
End Page:2737
Copyright:© Springer
Review Status:not specified
Audience:Experts Only
Abstract / Description:In the sixties James Watson suggested a twosite model for the ribosome comprising the P site for the peptidyl transfer RNA (tRNA) before peptide-bond formation and the A site, where decoding takes place according to the codon exposed there. In the eighties a third tRNA binding site was detected, the E site, which was specific for deacylated tRNA and turned out to be a universal feature of ribosomes. However, despite having three tRNA binding sites, only two tRNAs occupy the ribosome at a time during protein synthesis: at the A and P sites before translocation (PRE state) and at the P and E sites after translocation (POST state). The importance of having two tRNAs in the POST state has been revealed during the last 25 years, showing that the E site contributes two fundamental features: (i) the fact that incorporation of a wrong amino acid is not harmful for the cell (only 1 in about 400 misincorporations destroys the function of a protein) stems from the presence of an E-tRNA; (ii) maintenance of the reading frame is one of the most remarkable achievements of the ribosome, essential for faithful translation of the genetic information. The presence of the POST state E-tRNA prevents
Free Keywords:Protein synthesis - tRNA binding sites - A site - E site - accuracy of protein synthesis - frameshift
Comment of the Author/Creator:Email: Nierhaus@molgen.mpg.de
External Publication Status:published
Document Type:Article
Communicated by:Ribosome Group
Affiliations:MPI für molekulare Genetik
Identifiers:ISSN:1420-682X
DOI:10.1007/s00018-006-6125-4
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