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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents



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ID: 305802.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Classification of AAA+ proteins.
Authors:Ammelburg, Moritz; Frickey, Tankred; Lupas, Andrei
Language:English
Date of Publication (YYYY-MM-DD):2006-10
Title of Journal:J Struct Biol
Volume:156
Issue / Number:1
Start Page:2
End Page:11
Sequence Number of Article:16828312
Review Status:not specified
Audience:Not Specified
Abstract / Description:AAA+ proteins form a large superfamily of P-loop ATPases involved in the energy-dependent unfolding and disaggregation of macromolecules. In a clustering study aimed at defining the AAA proteins within this superfamily, we generated a map of AAA+ proteins based on sequence similarity, which suggested higher-order groups. A classification based primarily on morphological characteristics, which was proposed at the same time, differed from the cluster map in several aspects, such as the position of RuvB-like helicases and the inclusion of divergent clades, such as viral SF3 helicases and plant disease resistance proteins (RFL1). Here, we establish the presence of an alpha-helical domain C-terminal to the ATPase domain (the C-domain) as characteristic for AAA+ proteins and re-evaluate all clades proposed to belong to this superfamily, based on this characteristic. We find that RFL1 and its homologs (APAF-1, CED-4, MalT, and AfsR) are AAA+ proteins and SF3 helicases are not. We also present a new and more comprehensive cluster map, which assigns a central position to RuvB and clarifies the relationships between the clades of the AAA+ superfamily.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
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