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          Institute: MPI für medizinische Forschung     Collection: Arbeitsgruppe Zimmermann     Display Documents



ID: 307558.0, MPI für medizinische Forschung / Arbeitsgruppe Zimmermann
Isotope selection in distance measurements between nitroxides
Translation of Title:Isotope selection in distance measurements between nitroxides
Authors:Jeschke, Gunnar; Zimmermann, Herbert; Godt, Adelheid
Language:English
Date of Publication (YYYY-MM-DD):2006-05-01
Title of Journal:Journal of Magnetic Resonance
Journal Abbrev.:J. Magn. Reson.
Volume:180
Issue / Number:1
Start Page:137
End Page:146
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Self−assembly of spin−labeled synthetic macromolecules or biomacromolecules can lead to structures that contain more than two nitroxide radicals. Label−to−label distance distributions are then poorly resolved since established electron paramagnetic resonance techniques for distance measurements cannot select between the different pairs of nitroxides. A separation into different contributions can be achieved by partially labeling the nitroxide radicals by 15N or by deuterium and applying pulse electron electron double resonance techniques. With 15N labeling, strong suppression of either the 14N or the 15N contribution can be achieved by suitable choices of the excitation bandwidths and frequencies of the observer subsequence and pump pulse and linear combination of data sets. With deuterium labeling, interactions between only the isotope−labeled nitroxides can be selected by a two−dimensional version of the four−pulse double electron electron resonance experiment. This selection is based on the deep electron spin echo envelope modulation of deuterated nitroxides.
Free Keywords:EPR; ELDOR; Spin labeling; Protein structure; ESEEM
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Arbeitsgruppe Herbert Zimmermann
Identifiers:LOCALID:6550
URI:http://www.sciencedirect.com/science?_ob=ArticleUR...
URI:http://www.sciencedirect.com/science?_ob=ArticleUR...
URI:http://www.sciencedirect.com/science?_ob=ArticleUR...
DOI:10.1016/j.jmr.2006.02.002
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