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          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents

ID: 309274.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Transcriptional census of 36 microdissected colorectal cancers yields a gene signature to distinguish UICC II and III
Authors:Groene, Joern; Mansmann, Ulrich; Meister, Reinhard; Staub, Eike; Roepcke, Stefan; Heinze, Maya; Klaman, Irina; Brümmendorf, Thomas; Hermann, Klaus; Loddenkemper, Christoph; Pilarsky, Christian; Mann, Benno; Adams, Hans-Peter; Buhr, Heinz Johannes; Rosenthal, André
Research Context:NGFN; Grant Number: 01GR0459
Date of Publication (YYYY-MM-DD):2006-05-23
Title of Journal:International Journal of Cancer
Journal Abbrev.:Int. J. Cancer
Issue / Number:8
Start Page:1829
End Page:1836
Copyright:© 2006 Wiley-Liss, Inc.
Review Status:not specified
Audience:Experts Only
Abstract / Description:UICC stage II and III colorectal cancers (CRC) differ fundamentally in prognosis and therapeutic concepts. To analyze differential gene expression between both stages and to establish a relationship between molecular background and clinical presentation, tumor material from 36 unselected consecutive patients presenting with sporadic CRC, 18 UICC stage II and 18 UICC stage III, were laser microdissected to separate epithelial tumor cells. Gene expression levels were measured using U133A Affymetrix gene arrays. Twelve CRC associated signal transduction pathways as well as all 22,000 probe sets were screened for differential gene expression. We identified a signature consisting of 45 probe sets that allowed discrimination between UICC stage II and stage III with a rate of correct classification of about 80%. The most distinctive elements in this signature were the gene GSTP-binding elongation factor (GSPT2) and the transcription factor HOXA9. Differential expression of these genes was confirmed by quantitative real-time polymerase chain reaction (p(HOXA9) = 0.04, p(GSTP2) = 0.02). Despite the reliability of the presented data, there was no substantial differential expression of genes in cancer-related pathways. However, the comparison with recently published data corroborates the 45 gene signature showing structural agreement in the direction of fold changes of gene expression levels for our set of genes chosen to discriminate between both stages
Free Keywords:colorectal cancer • gene expression analysis • gene signature • classification • UICC stage II • UICC stage III
Comment of the Author/Creator:Correspondence to Joern Groene, Department of General, Vascular and Thoracic Surgery, Campus Benjamin Franklin, Charité Universitaetsmedizin, Hindenburgdamm 30, D-12200 Berlin, Germany
External Publication Status:published
Document Type:Article
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Department of General, Vascular and Thoracic Surgery, Campus Benjamin Franklin, Charité Universitaetsmedizin, Berlin, Germany;
2.Institute for Medical Informatics, Biometry, and Epidemiology, Ludwig Maximillians University München, München, Germany;
3.Department II, University of Applied Sciences, Berlin, Germany;
4.Department of Pathology, HELIOS Klinikum Emil von Behring, Berlin, Germany;
5.Novartis Institutes for Biomedical Research, Basel, Switzerland;
6.Signature Diagnostics AG, Voltaireweg 4B, Potsdam, Germany;
7.Institute of Pathology, Campus Benjamin Franklin, Charité Universitaetsmedizin, Berlin, Germany;
8.Department of Visceral, Thoracic and Vascular Surgery, University Hospital Dresden, Dresden, Germany;
9.Department of Surgery, Augusta-Kranken-Anstalt GmbH, Bochum, Germany.
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