Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2006     Display Documents



ID: 311159.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2006
Migration of zebrafish primordial germ cells: a role for myosin contraction and cytoplasmic flow
Authors:Blaser, Heiko; Reichman-Fried, Michal; Castanon, Irinka; Dumstrei, Karin; Marlow, Florence L; Kawakami, Koichi; Solnica-Krezel, Lilianna; Heisenberg, Carl-Philipp; Raz, Erez
Date of Publication (YYYY-MM-DD):2006
Title of Journal:Developmental Cell
Volume:11
Issue / Number:5
Start Page:613
End Page:627
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The molecular and cellular mechanisms governing cell motility and directed migration in response to the chemokine SDF-1 are largely unknown. Here, we demonstrate that zebrafish primordial germ cells whose migration is guided by SDF-1 generate bleb-like protrusions that are powered by cytoplasmic flow. Protrusions are formed at sites of higher levels of free calcium where activation of myosin contraction occurs. Separation of the acto-myosin cortex from the plasma membrane at these sites is followed by a flow of cytoplasm into the forming bleb. We propose that polarized activation of the receptor CXCR4 leads to a rise in free calcium that in turn activates myosin contraction in the part of the cell responding to higher levels of the ligand SDF-1. The biased formation of new protrusions in a particular region of the cell in response to SDF-1 defines the leading edge and the direction of cell migration.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:810
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.