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          Institute: MPI für molekulare Genetik     Collection: Department of Computational Molecular Biology     Display Documents

ID: 311430.0, MPI für molekulare Genetik / Department of Computational Molecular Biology
Nonribosomal peptide synthesis in S. pombe and the architecture of ferrichrome-type siderophore synthetases in fungi
Authors:Schwecke,, Torsten; Göttling, Kirsten; Durek, Pawel; Dueñas, Ines; Käufer, Norbert F.; Zock-Emmenthal, Susanne; Staub, Eike; Neuhof, Torsten; Dieckmann, Ralf; von Döhren, Hans
Date of Publication (YYYY-MM-DD):2006-02-27
Title of Journal:ChemBioChem : A European Journal of Chemical Biology
Journal Abbrev.:ChemBioChem
Issue / Number:4
Start Page:612
End Page:622
Copyright:© 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Review Status:not specified
Audience:Experts Only
Abstract / Description:A nonribosomal peptide synthetase (NRPS) in Schizosaccharomyces pombe, which possesses an unusual structure incorporating three adenylation domains, six thiolation domains and six condensation domains, has been shown to produce the cyclohexapeptide siderophore ferrichrome. One of the adenylation domains is truncated and contains a distorted key motif. Substrate-binding specificities of the remaining two domains were assigned by molecular modelling to glycine and to N-acetyl-N-hydroxy-L-ornithine. Hexapeptide siderophore synthetase genes of Magnaporthe grisea and Fusarium graminearum were both identified and analyzed with respect to substrate-binding sites, and the predicted product ferricrocin was identified in each. A comparative analysis of these synthetase systems, including those of the basidiomycete Ustilago maydis, the homobasidiomycete Omphalotus olearius and the ascomycetes Aspergillus nidulans, Aspergillus fumigatus, Fusarium graminearum, Cochliobolus heterostrophus, Neurospora crassa and Aureobasidium pullulans, revealed divergent domain compositions with respect to their number and positioning, although all produce similar products by iterative processes. A phylogenetic analysis of both NRPSs and associated L-N5-ornithine monooxygenases revealed that ferrichrome-type siderophore biosynthesis has coevolved in fungi with varying in trans interactions of NRPS domains.
Free Keywords:biosynthesis • evolution • ferrichrome • fungi • nonribosomal peptide synthetases
Comment of the Author/Creator:Correspondence to Norbert F. Käufer, Institute of Genetics, University of Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany, Fax: (+49) 531-391-5774

Correspondence to Hans von Döhren, Institute of Chemistry, Technical University Berlin, Franklinstrasse 29, 10587 Berlin, Germany, Fax: (+49) 30-314-22697

Supporting information for this article is available on the WWW under or from the author.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Martin Vingron
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Institute of Chemistry, Technical University Berlin, Franklinstrasse 29, 10587 Berlin, Germany;
2.Institute of Genetics, University of Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany;
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