MPI für molekulare Genetik / Department of Computational Molecular Biology |
|A genome-wide map of aberrantly expressed chromosomal islands in colorectal cancer|
|Authors:||Staub, Eike; Gröne, Jörn; Mennerich, Detlev; Röpcke, Stefan; Klamann, Irina; Hinzmann, Bernd; Castanos-Velez, Esmeralda; Mann, Benno; Pilarsky, Christian; Brümmendorf, Thomas; Weber, Birgit; Buhr, Heinz-Johannes; Rosenthal, André|
|Date of Publication (YYYY-MM-DD):||2006-09-18|
|Title of Journal:||Molecular Cancer|
|Journal Abbrev.:||Mol. Cancer|
|Copyright:||© 2006 Staub et al; licensee BioMed Central Ltd.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
|Review Status:||not specified|
|Abstract / Description:||Background:
Cancer development is accompanied by genetic phenomena like deletion and amplification of chromosome parts or alterations of chromatin structure. It is expected that these mechanisms have a strong effect on regional gene expression.
We investigated genome-wide gene expression in colorectal carcinoma (CRC) and normal epithelial tissues from 25 patients using oligonucleotide arrays. This allowed us to identify 81 distinct chromosomal islands with aberrant gene expression. Of these, 38 islands show a gain in expression and 43 a loss of expression. In total, 7.892 genes (25.3% of all human genes) are located in aberrantly expressed islands. Many chromosomal regions that are linked to hereditary colorectal cancer show deregulated expression. Also, many known tumor genes localize to chromosomal islands of misregulated expression in CRC.
An extensive comparison with published CGH data suggests that chromosomal regions known for frequent deletions in colon cancer tend to show reduced expression. In contrast, regions that are often amplified in colorectal tumors exhibit heterogeneous expression patterns: even show a decrease of mRNA expression. Because for several islands of deregulated expression chromosomal aberrations have never been observed, we speculate that additional mechanisms (like abnormal states of regional chromatin) also have a substantial impact on the formation of co-expression islands in colorectal carcinoma.
|Comment of the Author/Creator:||The electronic version of this article is the complete one and can be found online at: http://www.molecular-cancer.com/content/5/1/37|
|External Publication Status:||published|
|Communicated by:||Martin Vingron|
|Affiliations:||MPI für molekulare Genetik|
|External Affiliations:||1.Dept. of General, Vascular and Thoracic Surgery, Charité – Campus Benjamin Franklin, Berlin, Germany;
2.Signature Diagnostics AG, Potsdam, Germany;
3.HELIOS Hospital Emil von Behring, Institute of Pathology, Berlin, Germany;
4.Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany;
5.Dept. of Visceral, Thoracic, and Vascular Surgery, University Hospital Carl Gustav Carus Dresden, Germany;
6.Department of Surgery, Augusta-Kranken-Anstalt GmbH, Bochum, Germany;
7.metaGen Pharmaceuticals i.L., Berlin, Germany.
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