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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 312531.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
An efficient and economic enhancer mix for PCR
Authors:Ralser, Markus; Querfurth, Robert; Warnatz, Hans-Jörg; Lehrach, Hans; Yaspo, Marie-Laure; Krobitsch, Sylvia
Language:English
Date of Publication (YYYY-MM-DD):2006-09-01
Title of Journal:Biochemical and Biophysical Research Communications (Orlando, FL)
Journal Abbrev.:Biochem Biophys Res Commun
Volume:347
Issue / Number:3
Start Page:747
End Page:751
Copyright:© 2006 Elsevier Inc.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Polymerase chain reaction (PCR) has become a fundamental technique in molecular biology. Nonetheless, further improvements of the existing protocols are required to broaden the applicability of PCR for routine diagnostic purposes, to enhance the specificity and the yield of PCRs as well as to reduce the costs for high-throughput applications. One known problem typically reported in PCR experiments is the poor amplification of GC-rich DNA sequences. Here we designed and tested a novel effective and low-cost PCR enhancer, a concentration-dependent combination of betaine, dithiothreitol, and dimethyl sulfoxide that broadly enhanced the quantitative and/or qualitative output of PCRs. Additionally, we showed that the performances of this enhancer mix are comparable to those of commercially available PCR additives and highly effective with different DNA polymerases. Thus, we propose the routine application of this PCR enhancer mix for low- and high-throughput experiments.
Free Keywords:Polymerase chain reaction; GC-rich sequence; Enhancer; Additive; Promoter PCR; Genomic PCR; PCR template; Taq DNA polymerase
Comment of the Author/Creator:e-mail: krobitsc@molgen.mpg.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
Identifiers:ISSN:0006-291X
DOI:10.1016/j.bbrc.2006.06.151
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