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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 312991.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Structure of the ribosome-bound cricket paralysis virus IRES RNA
Authors:Schüler, Martin; Connell, Sean R.; Lescoute, Aurelie; Giesebrecht, Jan; Dabrowski, Marylena; Schroeer, Birgit; Mielke, Thorsten; Penczek, Pawel A.; Westhof, Eric; Spahn, Christian M. T.
Date of Publication (YYYY-MM-DD):2006-12
Title of Journal:Nature Structural & Molecular Biology
Journal Abbrev.:Nat Struct Mol Biol
Issue / Number:12
Start Page:1092
End Page:1096
Copyright:© 2006 Nature Publishing Group
Review Status:not specified
Audience:Experts Only
Abstract / Description:Internal ribosome entry sites (IRESs) facilitate an alternative, end-independent pathway of translation initiation. A particular family of dicistroviral IRESs can assemble elongation-competent 80S ribosomal complexes in the absence of canonical initiation factors and initiator transfer RNA. We present here a cryo-EM reconstruction of a dicistroviral IRES bound to the 80S ribosome. The resolution of the cryo-EM reconstruction, in the subnanometer range, allowed the molecular structure of the complete IRES in its active, ribosome-bound state to be solved. The structure, harboring three pseudoknot-containing domains, each with a specific functional role, shows how defined elements of the IRES emerge from a compactly folded core and interact with the key ribosomal components that form the A, P and E sites, where tRNAs normally bind. Our results exemplify the molecular strategy for recruitment of an IRES and reveal the dynamic features necessary for internal initiation.
Comment of the Author/Creator:e-mail: christian.spahn@charite.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Institut für Medizinische Physik und Biophysik, Charite-Universitätsmedizin Berlin, Ziegelstrasse 5-9, 10117-Berlin, Germany;
Architecture et Réactivité de l'ARN, Université Louis Pasteur, Institut de Biologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, 15 rue R. Descartes, F-67084 Strasbourg, France;
The University of Texas-Houston Medical School, 6431 Fannin, Houston, Texas 77030, USA
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