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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 312999.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Differential binding studies applying functional protein microarrays and surface plasmon resonance
Authors:Seitz, Harald; Hutschenreiter, Silke; Hultschig, Claus; Zeilinger, Carsten; Zimmermann, Bastian; Kleinjung, Frank; Schuchhardt, Johannes; Eickhoff, Holger; Herberg, Friedrich W.
Language:English
Date of Publication (YYYY-MM-DD):2006-10
Title of Journal:PROTEOMICS
Volume:6
Issue / Number:19
Start Page:5132
End Page:5139
Copyright:© 1999-2007 John Wiley & Sons, Inc.
Review Status:not specified
Audience:Experts Only
Abstract / Description:A variety of different in vivo and in vitro technologies provide comprehensive insights in protein-protein interaction networks. Here we demonstrate a novel approach to analyze, verify and quantify putative interactions between two members of the S100 protein family and 80 recombinant proteins derived from a proteome-wide protein expression library. Surface plasmon resonance (SPR) using Biacore technology and functional protein microarrays were used as two independent methods to study protein-protein interactions. With this combined approach we were able to detect nine calcium-dependent interactions between Arg-Gly-Ser-(RGS)-His6 tagged proteins derived from the library and GST-tagged S100B and S100A6, respectively. For the protein microarray affinity-purified proteins from the expression library were spotted onto modified glass slides and probed with the S100 proteins. SPR experiments were performed in the same setup and in a vice-versa approach reversing analytes and ligands to determine distinct association and dissociation patterns of each positive interaction. Besides already known interaction partners, several novel binders were found independently with both detection methods, albeit analogous immobilization strategies had to be applied in both assays.
Free Keywords:Binding studies; Protein microarray; Protein-protein interaction; S100 proteins; Surface plasmon resonance studies
Comment of the Author/Creator:e-mail: Harald Seitz (seitz@molgen.mpg.de)
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Universität Kassel, FB Biochemie, Kassel, Germany;
Biaffin GmbH & CoKG, Kassel, Germany;
MicroDiscovery GmbH., Berlin, Germany;
Scienion AG, Berlin, Germany
Identifiers:ISSN:1615-9853
DOI:10.1002/pmic.200500181
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