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          Institute: MPI für Biochemie     Collection: Structural Biology (W. Baumeister)     Display Documents



ID: 318480.0, MPI für Biochemie / Structural Biology (W. Baumeister)
Evaporated carbon stabilizes thin, frozen-hydrated specimens
Authors:Jakubowski, U.; Baumeister, W.; Glaeser, R. M.
Date of Publication (YYYY-MM-DD):1989
Title of Journal:Ultramicroscopy
Volume:31
Issue / Number:4
Start Page:351
End Page:356
Audience:Not Specified
Abstract / Description:A relatively simple modification has been designed and built for a commercial freeze-fracture machine which makes it possible to evaporate carbon onto frozen-hydrated specimens after the specimen grid has been mounted into a side-entry electron microscope cryo-holder. The evaporated carbon film greatly improves the stability of self-supported, vitreous ice films during exposure to the electron beam. Specimen charging is also greatly reduced. As a consequence, the image resolution attainable with carbon-coated, vitreous ice specimens appears to be limited only by the mechanical stability of the cryostage itself. (13 References).
Free Keywords:Carbon; Electron microscopy; Specimen preparation; Sample preparation [A0660E]; Electron and ion microscopes and techniques [A0780]; Thin film growth, structure, and epitaxy [A6855]
External Publication Status:published
Document Type:Article
Communicated by:Anton Hillebrand
Affiliations:MPI für Biochemie/Structural Biology (W. Baumeister)/
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