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          Institute: MPI für Biochemie     Collection: Structural Biology (W. Baumeister)     Display Documents



ID: 318505.0, MPI für Biochemie / Structural Biology (W. Baumeister)
Interfacial energies and surface-tension forces involved in the preparation of thin, flat crystals of biological macromolecules for high-resolution electron microscopy
Authors:Glaeser, R. M.; Zilker, A.; Radermacher, M.; Gaub, H. E.; Hartmann, T.; Baumeister, W.
Date of Publication (YYYY-MM-DD):1991
Title of Journal:Journal of Microscopy
Volume:161
Issue / Number:1
Start Page:21
End Page:45
Audience:Not Specified
Abstract / Description:Data collection by electron diffraction and electron microscopy at high resolution and high tilt angles requires thin crystals of biological macromolecules that are flat to at least 1 degrees , and perhaps less than 0.2 degrees , over areas as large as 1 mu m/sup 2/ or more. In addition to determining empirically by electron diffraction experiments whether sufficiently flat specimens can be prepared on various types of modified or unmodified carbon support films, the authors have begun to use other techniques to characterize both the surfaces involved and the interaction of the specimen with these surfaces. In the specific case of large, monolayer crystals of bacteriorhodopsin prepared as glucose-embedded specimens on hydrophobic carbon films, it was concluded that the initial interfacial interaction involves adsorption of the specimen to the air-water interface rather than adsorption of the specimen to the substrate. (62 References).
Free Keywords:Biological specimen preparation; Electron microscope examination of materials; Molecular biophysics; Proteins; Surface energy; Surface tension; Interactions with radiations at the biomolecular level [A8715M]; Biophysical instrumentation and techniques [A8780]
External Publication Status:published
Document Type:Article
Communicated by:Anton Hillebrand
Affiliations:MPI für Biochemie/Structural Biology (W. Baumeister)/
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