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          Institute: MPI für Biochemie     Collection: Structural Biology (W. Baumeister)     Display Documents

ID: 318613.0, MPI für Biochemie / Structural Biology (W. Baumeister)
Hydration-scanning tunneling microscopy as a reliable method for imaging biological specimens and hydrophilic insulators
Authors:Heim, M.; Eschrich, R.; Hillebrand, A.; Knapp, H. F.; Cevc, G.; Guckenberger, R.
Publisher:Scanning Microscopy Intl
Date of Publication (YYYY-MM-DD):1996
Title of Book:The Science of Biological Specimen Preparation for Microscopy: Proceedings of the 14th Pfefferkorn Conference, Belleville, Il
Start Page:73
End Page:80
Full Name of Book-Editor(s):Malecki, Marek
Title of Series:Scanning Microscopy Supplement
Audience:Not Specified
Abstract / Description:The recently discovered high lateral conductivity of molecularly thin adsorbed water films enables investigation of biological specimens, and even of surfaces of hydrophilic insulators by scanning tunneling microscopy (STM). Here we demonstrate the capabilities of this method, which we call hydration-STM (HSTM), with images of various specimens taken in humid atmosphere: We obtained images of a glass coverslip, collagen molecules, tobacco mosaic virus, lipid bilayers and cryosectioned bovine achilles tendon on mica. To elucidate the physical mechanism of this conduction phenomenon we recorded current-voltage curves on hydrated mica. This revealed a basically ohmic behavior of the I-V curves without a threshold voltage to activate the current transport and indicates that electrochemistry probably does not dominate the surface conductivity. We assume that the conduction mechanism is due to structuring of water at the surface. [References: 19]
Free Keywords:Surface conductivity; Structured water; Thin films; Surface electrochemistry; Glass; Collagen; Bovine achilles tendon; Lipid layers.; Water-structure; Wet insulators; Electrochemistry; Conductivity; Films; Stm; Membranes; Molecules; Surface; Tip.; Current Book Contents in Current Contents(R)/Life Sciences.
External Publication Status:published
Document Type:InBook
Communicated by:Anton Hillebrand
Affiliations:MPI für Biochemie/Structural Biology (W. Baumeister)/
External Affiliations:Reprint available from: Heim M Max Planck Inst Biochem, Abt Mol Strukt Biol D-82151 Martinsried Germany Max Planck Inst Biochem, Abt Mol Strukt Biol D-82151 Martinsried Germany
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