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          Institute: MPI für Biochemie     Collection: Structural Biology (W. Baumeister)     Display Documents

ID: 318626.0, MPI für Biochemie / Structural Biology (W. Baumeister)
The fla gene cluster is involved in the biogenesis of flagella in Halobacterium salinarum
Authors:Patenge, N.; Berendes, A.; Engelhardt, H.; Schuster, S. C.; Oesterhelt, D.
Date of Publication (YYYY-MM-DD):2001
Title of Journal:Molecular Microbiology
Journal Abbrev.:Mol. Microbiol
Issue / Number:3
Start Page:653
End Page:663
Audience:Not Specified
Abstract / Description:In this study, a flagella-related protein gene cluster is described for Halobacterium salinarum. The fla gene cluster is located upstream of the flagellin genes flgB1-3 and oriented in the opposite direction. It consists of nine open reading frames (ORFs): htpIX, a member of the halobacterial transducer protein gene family, and the genes flaD-K. The genes flaD, E, G, H, I and J share high homologies with genes from other Archaea. Interestingly, flaK shows similarities to bacterial genes involved in the regulation of flagellar synthesis. The ORFs of flaH, flal and flaK contain sequences coding for nucleotide binding sites. Furthermore, flal contains a motif called the bacterial type II secretion protein E signature, indicating a functional relation to members of the bacterial pili type IV-type II secretion protein superfamily. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the genes flaE to flaK are transcribed into one polycistronic message. In frame deletion mutants of flal were generated by gene replacement. The deletion strain lacks motility and belongs to the fla(-) mutant class, indicating that it is deficient in flagellar biogenesis. The overall amount of flagellin protein in Delta flal cells is reduced, although transcription of the flagellin genes is unaffected. Therefore, the flal gene product is involved in the biosynthesis, transport or assembly of flagella in H. salinarum. [References: 62]
Free Keywords:Complete genome sequence; Pseudomonas-aeruginosa; Twitching motility; Myxococcus-xanthus; Methanococcus-voltae; Escherichia-coli; Multigene family; Type-4 fimbriae; Proteins; Archaeon.; Microbiology in Current Contents(R)/Life Sciences.
External Publication Status:published
Document Type:Article
Communicated by:Anton Hillebrand
Affiliations:MPI für Biochemie/Structural Biology (W. Baumeister)/
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