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          Institute: MPI für Biochemie     Collection: Structural Biology (W. Baumeister)     Display Documents



ID: 318709.0, MPI für Biochemie / Structural Biology (W. Baumeister)
Expression of an archaeal chaperonin in e-coli - formation of homo- (alpha, beta) and hetero-oligomeric (alpha+beta) thermosome complexes
Authors:Waldmann, T.; Nitsch, M.; Klumpp, M.; Baumeister, W.
Date of Publication (YYYY-MM-DD):1995-11-27
Title of Journal:FEBS Letters
Volume:376
Issue / Number:1-2
Start Page:67
End Page:73
Audience:Not Specified
Abstract / Description:Go-expression of the two genes encoding the alpha- and beta-subunit of the Thermoplasma acidophilum thermosome in Escher ichia coli yielded fully assembled hetero-oligomeric complexes (alpha + beta). Surprisingly, also separate expression of both genes resulted in formation of hexadecameric complexes (alpha, beta) in the bacterial cytoplasm. On electron micrographs these complexes were indistinguishable from each other and from the native thermosome. The recombinant alpha-complex as well as the native thermosome could be reconstituted in vitro from their dissociated subunits in the presence of Mg-ATP. [References: 28]
Free Keywords:Thermosome; Molecular chaperone; Gene expression; Archaebacteria; Thermoplasma acidophilum; Electron microscopy.; Thermophilic archaebacterium; Escherichia-coli; Rna-polymerase; Polypeptide-1; Subunits; System; Genes.; Biochemistry & biophysics.
External Publication Status:published
Document Type:Article
Communicated by:Anton Hillebrand
Affiliations:MPI für Biochemie/Structural Biology (W. Baumeister)/
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