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          Institute: MPI für Biochemie     Collection: Structural Biology (W. Baumeister)     Display Documents



ID: 318718.0, MPI für Biochemie / Structural Biology (W. Baumeister)
Electron tomography of single ice-embedded macromolecules - three-dimensional alignment and classification
Authors:Walz, J.; Typke, D.; Nitsch, M.; Koster, A. J.; Hegerl, R.; Baumeister, W.
Date of Publication (YYYY-MM-DD):1997-12
Title of Journal:Journal of Structural Biology
Volume:120
Issue / Number:3
Start Page:387
End Page:395
Audience:Not Specified
Abstract / Description:From 3-D reconstructions of automatically recorded tilt series of ice-embedded macromolecules, several hundred 3-D images of single particles can be extracted. Here we describe correlation-based techniques to align the particles with respect to translation and orientation in 3-D and the calculation of an averaged reconstruction after application of the correct weighting function to the particle projections. Multivariate statistical analysis and classification are applied to the set of three-dimensionally reconstructed particles to investigate interimage variations on the 3-D level. (C) 1997 Academic Press. [References: 24]
Free Keywords:Escherichia-coli; Biological particles; Ribosome; Reconstruction; Micrographs; Microscopy; Resolution; Images.; Biochemistry & biophysics.
External Publication Status:published
Document Type:Article
Communicated by:Anton Hillebrand
Affiliations:MPI für Biochemie/Structural Biology (W. Baumeister)/
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