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          Institute: MPI für Evolutionsbiologie     Collection: Ecophysiology     Display Documents



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ID: 319798.0, MPI für Evolutionsbiologie / Ecophysiology
Ammonia-oxidizing β-Proteobacteria from the oxygen minimum zone off northern Chile
Authors:Molina, Verónica; Ulloa, Osvaldo; Farías, Laura; Urrutia, Homero; Ramírez, Salvador; Junier, Pilar; Witzel, Karl-Paul
Language:English
Date of Publication (YYYY-MM-DD):2007-06
Title of Journal:Applied and Environmental Microbiology
Journal Abbrev.:Appl. Environ. Microbiol.
Volume:73
Issue / Number:11
Start Page:3547
End Page:3555
Review Status:not specified
Audience:Not Specified
Abstract / Description:The composition of ammonia-oxidizing bacteria from the β-Proteobacteria subclass (βAOB) was studied in the surface and upper-oxycline oxic waters (2- to 50-m depth, similar to 200 to 44 mu M O-2) and within the oxygen minimum zone (OMZ) suboxic waters (50- to 400-m depth, <= 10 mu M O-2) of the eastern South Pacific off northern Chile. This study was carried out through cloning and sequencing of genes coding for 16S rRNA and the ammonia monooxygenase enzyme active subunit (amoA). Sequences affiliated with Nitrosospira-like cluster 1 dominated the 16S rRNA gene clone libraries constructed from both oxic and suboxic waters. Cluster 1 consists exclusively of yet-uncultivated beta AOB from marine environments. However, a single clone, out of 224 obtained from the OMZ, was found to belong to Nitrosospira lineage cluster 0. To our knowledge, cluster 0 sequences have been derived from βAOB isolated only from sand, soil, and freshwater environments. Sequences in clone libraries of the amoA gene from the surface and upper oxycline could be grouped in a marine subcluster, also containing no cultured representatives. In contrast, all 74 amoA sequences originating from the OMZ were either closely affiliated with cultured Nitrosospira spp. from clusters 0 and 2 or with other yet-uncultured βAOB from soil and an aerated-anoxic Orbal process waste treatment plant. Our results reveal the presence of Nitrosospira-like βAOB in both oxic and suboxic waters associated with the OMZ but with a clear community shift at the functional level (amoA) along the strong oxygen gradient.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Limnologie/Abt. Ökophysiologie
External Affiliations:Univ Concepcion, Dept Oceanog, Lab Proc Oceanog & Clima, PROFC Cabina 7,Casilla 160-C, Concepcion, Chile; Univ Concepcion, Dept Microbiol, Concepcion, Chile
Identifiers:ISSN:0099-2240 [ID-No:1]
DOI:10.1128/AEM.02275-06 [ID-No:2]
LOCALID:2565/S 38715 [Listen-Nummer/S-Nummer]
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