Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Dynamik komplexer technischer Systeme     Collection: Bioprocess Engineering     Display Documents



  history
ID: 327741.0, MPI für Dynamik komplexer technischer Systeme / Bioprocess Engineering
Glutamine-free media for vaccine production processes
Authors:Genzel, Y.; Alt, R.; Reichl, U.
Language:English
Publisher:Springer
Date of Publication (YYYY-MM-DD):2003
Title of Proceedings:Proceedings ot the 18th ESACT meeting : Animal Cell Technology meets Genomics
Start Page:767
End Page:770
Name of Conference/Meeting:ESACT 2003
Place of Conference/Meeting:Granada, Spain
(Start) Date of Conference/Meeting
 (YYYY-MM-DD):
2003-05-11
End Date of Conference/Meeting 
 (YYYY-MM-DD):
2003-05-14
Review Status:not specified
Audience:Not Specified
Intended Educational Use:No
Abstract / Description:In cell culture technology high ammonia concentrations can limit cell growth and product formation, especially when focussing on higher cell densities. This high ammonia concentration normally results from glutamine consumption and decomposition. To overcome such problems we have tested several options and developed a glutamine-free medium for cell lines used in industrial applications.
Preliminary investigations were carried out in static cultures of BHK 21, CHO and MDCK cells.
Detailed characterization of MDCK cell metabolism was done for a vaccine production process of influenza virus (equine influenza Newmarket 1/93 H3N8) in MDCK cells using this newly developed glutamine-free medium: The results for cell growth and virus replication in a large-scale controlled microcarrier culture (5L bioreactor) are compared to the production in glutamine containing (2 mM) GMEM medium. Amino acid uptake and release will be compared with other on- & off-line data, to evaluate the importance of some amino acids in different phases of cell metabolism and virus replication with respect to lactate and ammonia production.
External Publication Status:published
Document Type:Conference-Paper
Communicated by:Udo Reichl
Affiliations:MPI für Dynamik komplexer technischer Systeme/Bioprocess Engineering
Identifiers:LOCALID:GenzelAltReichl2005
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.