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          Institute: MPI für Infektionsbiologie     Collection: RNA Biology     Display Documents



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ID: 331484.0, MPI für Infektionsbiologie / RNA Biology
Characterization of the role of ribonucleases in Salmonella small RNA decay.
Authors:Viegas, Sandra C.; Pfeiffer, Verena; Sittka, Alexandra; Silva, Ines J.; Vogel, Jörg; Arraiano, Cecilia M.
Language:English
Date of Publication (YYYY-MM-DD):2007-11-03
Title of Journal:Nucleic Acids Research
Volume:35
Issue / Number:22
Start Page:7651
End Page:7664
Copyright:© 2007 The Author(s)
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Review Status:not specified
Audience:Experts Only
Abstract / Description:In pathogenic bacteria, a large number of sRNAs coordinate adaptation to stress and expression of virulence genes. To better understand the turnover of regulatory sRNAs in the model pathogen, Salmonella typhimurium, we have constructed mutants for several ribonucleases (RNase E, RNase G, RNase III, PNPase) and Poly(A) Polymerase I. The expression profiles of four sRNAs conserved among many enterobacteria, CsrB, CsrC, MicA and SraL, were analysed and the processing and stability of these sRNAs was studied in the constructed strains. The degradosome was a common feature involved in the turnover of these four sRNAs. PAPI-mediated polyadenylation was the major factor governing SraL degradation. RNase III was revealed to strongly affect MicA decay. PNPase was shown to be important in the decay of these four sRNAs. The stability of CsrB and CsrC seemed to be independent of the RNA chaperone, Hfq, whereas the decay of SraL and MicA was Hfq-dependent. Taken together, the results of this study provide initial insight into the mechanisms of sRNA decay in Salmonella, and indicate specific contributions of the RNA decay machinery components to the turnover of individual sRNAs.
Comment of the Author/Creator:ACKNOWLEDGEMENTS
The authors would like to thank J. Andrade for critical reading of this manuscript and M. G. Conde for technical assistance. S.C.V. was recipient of a Fundação para a Ciência e Tecnologia (FCT) Doctoral Fellowship (SFRH/BD/6479/2001) and I.J.S. was recipient of a FCT research fellowship. Funding for this work was partially provided by a Portuguese-German joint program to C.M.A. and J.V. (GRICES-DAAD). The work at the Instituto de Tecnologia Química e Biológica was supported by FCT (Portugal). Funding to pay the Open Access publication charges for this article was provided by Fundação para a Ciência e a Tecnologia, Portugal.

Conflict of interest statement. None declared.
External Publication Status:published
Document Type:Article
Communicated by:Hilmar Fünning
Affiliations:MPI für Infektionsbiologie/RNA Biology
External Affiliations:Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Apartado 127, 2781-901 Oeiras, Portugal
Identifiers:ISSN:0301-5610
DOI:10.1093/nar/gkm916
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