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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents



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ID: 331533.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
A CTP-Dependent Archaeal Riboflavin Kinase Forms a Bridge in the Evolution of Cradle-Loop Barrels.
Authors:Ammelburg, Moritz; Hartmann, Markus; Djuranovic, Sergej; Alva, Vikram; Koretke, K.; Martin, Joerg; Sauer, Guido; Truffault, Vincent; Zeth, Kornelius; Lupas, Andrei; Coles, Murray
Language:English
Date of Publication (YYYY-MM-DD):2007-12
Title of Journal:Structure
Volume:15(12)
Start Page:1577
End Page:1590
Sequence Number of Article:18073108
Review Status:not specified
Audience:Not Specified
Abstract / Description:Proteins of the cradle-loop barrel metafold are formed by duplication of a conserved betaalphabeta-element, suggesting a common evolutionary origin from an ancestral group of nucleic acid-binding proteins. The basal fold within this metafold, the RIFT barrel, is also found in a wide range of enzymes, whose homologous relationship with the nucleic acid-binding group is unclear. We have characterized a protein family that is intermediate in sequence and structure between the basal group of cradle-loop barrels and one family of RIFT-barrel enzymes, the riboflavin kinases. We report the structure, substrate-binding mode, and catalytic activity for one of these proteins, Methanocaldococcus jannaschii Mj0056, which is an archaeal riboflavin kinase. Mj0056 is unusual in utilizing CTP rather than ATP as the donor nucleotide, and sequence conservation in the relevant residues suggests that this is a general feature of archaeal riboflavin kinases.
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
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